Tri(tridecyl) benzene-1,2,4-tricarboxylate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

EC Number:

302-446-4

EC Name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

Cas Number:

94109-09-8

Molecular formula:

C48H84O6

IUPAC Name:

tri(tridecyl) benzene-1,2,4-tricarboxylate

Test material form:

liquid

Details on test material:

- Moisture content: <= 0.1%

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot P-2960F-10
- Expiration date of the lot/batch: June 29, 2013
- Purity test date: > 99%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: Stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAF (wateraccomodated fractions) due to insolubility of the test material
- Eluate: water
-- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): To prepare the WAF, 0.1001 g of the test item was weighted onto a watch glass. Dilution water (approximately 100 mL) was used to rinse the test item off the watch glasss into a 2 L beaker which contaied approximately 600 mL of dilution water. The total volume was brought to 1 L in the beaker using dilution water. A WAF control was prepared in teh sme way with the xeception that no test item was added to the 1 L of dilution water.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapita
- Strain: CC Strain 37
- Source (laboratory, culture collection): obtained from the Canadian Phycological Culture Centre, Waterloo, ON , Canada, as a sterile liquid starter culture.
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: The algae were cultured in 250 mL glass Erlenmeyer flasks, using Nutrient Medium and was based on the procedures in the Test Method OECD 201: Alga, Growth Inhibition (DCN80-C-017)

ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): see the report
- Any deformed or abnormal cells observed: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

23°C

pH:

7.4 to 7.5 (initial) - 7.9 to 8.0 (final)

Conductivity:

92 to 94 (initial) microS/cm

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open / closed : closed
- Material, size, headspace, fill volume: 50 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 1.02 X 104 cells/mL
- Control end cells density:
- No. of organisms per vessel:6
- No. of vessels per concentration (replicates):6
- No. of vessels per control (replicates):6
- No. of vessels per vehicle control (replicates):6

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: WAF
- Conductivity: 92 to 94 at 0h microS/cm
- Culture medium different from test medium: none

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod:24h light
- Light intensity and quality: 6461-8241 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] : counting chamber
- Other: Conductivity and pH

TEST CONCENTRATIONS
- Spacing factor for test concentrations: WAF 100 mg/L
- Justification for using less concentrations than requested by guideline: insolubility of the test item

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations of the test material, using both Specific Growth Rate and Cell Yield, the estimated 72h IC50, IC25 and the LOEC (Lowest Observed Effect Concentration) were > 100 mg/L.
The NOEC (No Observed Effect Concentration) was 100 mg/L.