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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
yes
Remarks:
please see below "principles of method if other than guideline"
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Principles of method if other than guideline:
Deviations from the Study Plan
The following deviations were observed:
• The temperature in the experiment was in the range 21.5 – 24.7 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and expo-nential growth was observed in the blank control this was stated as uncritical.
• The temperature during pre-culture incubation was in the range 19.7 - 22.5 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and exponential growth was observed in the blank control this was stated as uncriti-cal. The deviations were assessed and signed by the study director on 15. May 2019. 13.2

Deviations from the Guideline
The following deviations were observed:
• The stock solutions to be autoclaved were autoclaved for 20 minutes instead of the required 15 minutes. This can be considered uncritical, as the goal of sterility is achieved.
• The temperature in the experiment was in the range 21.5 – 24.7 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and expo-nential growth was observed in the blank control this was stated as uncritical.
• The temperature during pre-culture incubation was in the range 19.7 – 22.5 °C, and therefore not in the desired range (21.0 - 24.0 °C). As all validity criteria were met and exponential growth was observed in the blank control this was stated as uncriti-cal
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl (9Z,12Z,15Z)-9,12,15-octadecatrienoate
EC Number:
206-102-3
EC Name:
Methyl (9Z,12Z,15Z)-9,12,15-octadecatrienoate
Cas Number:
301-00-8
Molecular formula:
C19H32O2
IUPAC Name:
methyl octadeca-9,12,15-trienoate
Constituent 2
Chemical structure
Reference substance name:
Methyl linoleate
EC Number:
203-993-0
EC Name:
Methyl linoleate
Cas Number:
112-63-0
Molecular formula:
C19H34O2
IUPAC Name:
methyl octadeca-9,12-dienoate
Constituent 3
Chemical structure
Reference substance name:
Methyl oleate
EC Number:
203-992-5
EC Name:
Methyl oleate
Cas Number:
112-62-9
Molecular formula:
C19H36O2
IUPAC Name:
methyl (Z)-octadec-9-enoate
impurity 1
Chemical structure
Reference substance name:
Methyl palmitate
EC Number:
203-966-3
EC Name:
Methyl palmitate
Cas Number:
112-39-0
Molecular formula:
C17H34O2
IUPAC Name:
methyl palmitate
Test material form:
liquid
Specific details on test material used for the study:
Batch no. 18010144
Appearance colourless to pale yellow liquid
Composition METHYL OLEATE [CAS 112-62-9] 17 - 23 %;
METHYL LINOLEATE [CAS 112-63-0] 12 - 20 %;
METHYL LINOLENATE [CAS 301-00-8] 47-53 %;
OTHER METHYL ESTERS 4 - 16 %
CAS No. not applicable
EC-No. 907-284-3
Purity GC - 50.25 % linolenate methyle
Homogeneity homogeneous
Production date 15. Feb. 2018
Expiry date 15. Feb. 2020
Storage Room Temperature (20 ± 5°C)
Specific gravity 0.8872

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
6 g NaCl was added to 100 mL test solution resp. blank control solution; then, the clear solution was extracted two times with the solvent dichloromethane (2 x 4 mL), the organic phase was collected into a flask after drying with Na2SO4, the solvent dichloromethane was evaporated at 40 °C and the residue was dissolved in 2 mL of 10 mg/L ISTD (internal standard) stock solution and the solution was measured via GC/MSD.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Unicellular freshwater green alga.
Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position Chlorophyta - Chlorophyceae


Origin and Culture
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
21.5 – 24.7 °C
pH:
7.7
Nominal and measured concentrations:
nominal concentration: 100mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Results and discussion

Effect concentrations
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The toxicity to aquatic algae and cyanobacteria was performed according to the OECD 101 guideline.

The test substance has the following EC50 72h: >100 mg/L.