Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

The ready biodegradability of Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, was assessed under aerobic conditions at a GLP accredited laboratory according to the OECD Guideline 301B, “Ready biodegradability; CO2 evolution test” (Best, 2018).


In an initial experiment conducted using a test item concentration equivalent to 10 mg carbon/L, the toxicity control vessel, containing both the test item and reference substance (sodium benzoate), showed <25% biodegradation after 14 days, indicating that the test item had inhibitory effects on the microbial inoculum activity. The definitive test therefore exposed activated sludge micro-organisms with mineral medium to a reduced test item concentration equivalent to 5 mg carbon/L. Tests were conducted in sealed culture vessels in the dark at temperatures between 22-24°C for 28 days.


The reference substance attained 79% biodegradation after 14 days, with >60% degradation within a 10-day window, thereby confirming the suitability of the inoculum and test conditions. All other validity criteria specified by the OECD 301B Guideline were also met.


The test item attained 0% biodegradation (based on CO2 evolution) after 28 days and is therefore concluded to be not readily biodegradable.

Additional information