Reaction mass of disodium 2-[[2-hydroxy-3-[[methyl-(2-oxido-2-oxoethyl)amino]methyl]-5-nonylphenyl]methyl-methylamino and sodium 2-[(2-hydroxy-5-nonylphenyl)methyl-methylamino]acetate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study currently in draft

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

NA (study was performed according to guidelines).

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

NA

Post exposure observation period:

NA

Salinity:

NA

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.

Sublethal observations / clinical signs:

Chemical analysis of test preparations

Nominal Concentration (mg/L)	0 hours (Fresh)		24 hours (Old)		72 hours (Fresh)		96 hours (Old)
	Measured Concentration (mg/L)	% of nominal	Measured Concentration (mg/L)	% of nominal	Measured Concentration (mg/L)	% of nominal	Measured Concentration (mg/L)	% of nominal
Control	<LOQ	-	<LOQ	-	<LOQ	-	<LOQ	-
3.2	3.04	95	2.68	84	2.91	91	5.11	160
100 – RO water	94.2	94	-	-	85.5	86	6.56	7
P. Rec (0.102)	0.0960	94	0.120	109	0.120	109	0.120	109
P. Rec (0.102)	0.0944	93	0.122	111	0.122	111	0.122	111

Conclusions:

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.

Executive summary:

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to fish was assessed at a GLP accredited laboratory according to the OECD Guideline 203 ("Fish, Acute Toxicity Test").

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC₅₀ value is >3.2 mg/L.

Description of key information

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to fish was assessed at a GLP accredited laboratory according to the OECD Guideline 203 ("Fish, Acute Toxicity Test").

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC₅₀ value is >3.2 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.2 mg/L