Reaction mass of disodium 2-[[2-hydroxy-3-[[methyl-(2-oxido-2-oxoethyl)amino]methyl]-5-nonylphenyl]methyl-methylamino and sodium 2-[(2-hydroxy-5-nonylphenyl)methyl-methylamino]acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study currently in draft

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

NA (study was performed according to guidelines).

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

NA

Post exposure observation period:

NA

Salinity:

NA

Nominal and measured concentrations:

Nominal test concentrations: 0.10, 0.32, 1.0, 3.2, 10 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effects of test item on Daphnia magna over a 48-hour exposure period 

Nominal Concentration (mg/l)	Percentage Immobilisation at 24 Hours (%)	Percentage Immobilisation at 48 Hours (%)
Control	5	5
0.10	10	10
0.32	5	5
1.0	0	0
3.2	0	25
10	85	100

Chemical analysis of test preparations 

Nominal Concentration (mg/l)	0 Hours		24 Hours (Old)		24 Hours (Fresh)		48 Hours (Old)
	Measured Concentration (mg/l)	% of Nominal	Measured Concentration (mg/l)	% of Nominal	Measured Concentration (mg/l)	% of Nominal	Measured Concentration (mg/l)	% of Nominal
Control	<LOQ	-	<LOQ	-	<LOQ	-	<LOQ	-
0.10	0.157	157	0.103	103	0.182	182	0.0775	78
0.32	0.569	178	0.372	116	0.784* / 0.326**	245* / 102**	0.502	157
1.0	1.63* / 0.935**	163* / 94**	1.23	123	1.77* / 0.967**	177* / 97**	1.05	105
3.2	4.04	126	3.15	98	4.36	136	3.23	101
10	13.3	133	9.70	97	16.2* / 10.5**	162* / 105**	11.0	110
10 (RO Water)	12.8	128	12.2	122	13.2	132	14.1	141
P. Rec 0.104					0.0997	96
P. Rec 0.104					0.102	98

*outside calibration range as actual concentration much higher than nominal concentrations

**duplicate samples stored prior to analysis

Conclusions:

Exposure of Daphnia magna to the test item over a 48-hour period resulted in an EC50 value of 3.7 mg/L based on immobilisation.

Executive summary:

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to the aquatic invertebrate Daphnia magna was assessed at a GLP accredited laboratory according to the OECD Guideline 202 ("Daphnia sp. Acute Immobilisation Test").

Exposure of test organisms to the test item over a 48-hour period resulted in an EC₅₀ value of 3.7 mg/L based on immobilisation.

Description of key information

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to the aquatic invertebrate Daphnia magna was assessed at a GLP accredited laboratory according to the OECD Guideline 202 ("Daphnia sp. Acute Immobilisation Test").

Exposure of test organisms to the test item over a 48-hour period resulted in an EC₅₀ value of 3.7 mg/L based on immobilisation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.7 mg/L