N,N'-(2-chloro-1,4-phenylene)bis(3-oxobutaneamide)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-12-06 to 2000-02-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

443-010-4

EC Name:

-

Cas Number:

53641-10-4

Molecular formula:

C14H15ClN2O4

IUPAC Name:

N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Taken from a municipal sewage treatment plant, filtered, and maintained in aerobic condition by aeration until test begin.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Reference substance

Reference substance:

other: Sodium acetate

Results and discussion

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

56

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
0 % degradation after 7 d
36 % degradation after 14 d
56 % degradation after 21 d
56 % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
92 % degradation after 7 d
100 % degradation after 14 d
100 % degradation after 21 d
100 % degradation after 28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

All validity criteria according to the guideline were met

Interpretation of results:

not readily biodegradable

Remarks:

Pass level of > 70 % biodegradation within a 10-day window was not reached

Executive summary:

The ready biodegradability in the modified OECD Screening Test was determined with a non-adapted activated sludge for the test item "Cl-DAEP" (batch number: 90003 CCL), over a period of 28 days. The study was conducted from December 07, 1999 to January 04, 2000 according to OECD 301 E in the Dr. U.Noack-Laboratorium für Angewandte Biologie. 

The test item was tested in a concentration of 50 mg/L in duplicates, corresponding to a TOC of 0.533 mg C/mg test item and a carbon content of 26.7 mg C/L in the test vessel.

The biodegradation of the test item was followed by the determination of DOC.

In order to check the activity of the study system sodium acetate in a concentration of 130 mg/L was used as functional control. The percentage degradation of the functional control reached the pass level of > 70 % after 1 day. After 14 days, a degradation rate of 1000 % was reached.

In the toxicity control containing both test and reference item, the biodegradation came to 48 % after 14 days. The test item can be assumed to be not inhibitory in the test.

The biodegradation of the test item was compared to a readily degradable functional control and a toxicity control. The test item in the test concentration of 50 mg/L reached the level of 10 % (begin of the biodegradation) after 9 days. In the 10-d-window a mean biodegradation rate of 50 % and after 21 days of 56 % and the plateau was reached. The 70 % level was not passed during the test. After 28 days the biodegradation rate came to 56 %.

The pass level of a biodegradation > 70 % was not reached in a 10-d-window. The test item must be regarded to be "not readily biodegradable" in the 10-d-window.

The validity criteria of the test are fulfilled according to the guideline.