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EC number: 701-305-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 July – 03 Sep 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pitch, coal tar, high-temp., < 1% 4- to 5-membered condensed ring aromatic hydrocarbons
- EC Number:
- 701-305-8
- Molecular formula:
- not applicaple - UVCB
- IUPAC Name:
- Pitch, coal tar, high-temp., < 1% 4- to 5-membered condensed ring aromatic hydrocarbons
- Test material form:
- solid: pellets
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for TOC-analysis were taken at 0 h and 72 h (TOC-Analyser DIMA-TOC 100).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Alga-medium
for 24 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The pitch material was reduced to small pieces with a pestle and then was directly weighed into the glass beakers.
The beakers were then filled with Alga-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 24 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.
- Pre-conditioning: The test vessels of the Definite Alga-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.
- Differential loading: control, 10, 25, 50, 100, 250, and 500 mg/L
- Controls: without test material (blank; Alga medium)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: unicellular green alga
- Strain: : SAG 86.81
- Source: Albrecht von Haller Institut für Pflanzenwissenschaften, Universität Göttingen
- Age of inoculum (at test initiation): ca. 72 h
[Pre-culture: Incubation in 250 mL Erlenmeyer flasks at ± 25 °C on a shaker
(80 - 120 rpm), ca. 72 h, ca. 10000 lux (Gro-Lux fluorescent tubes), permanent illumination]
- Method of cultivation: Sterile on agar tubes (OECD-Medium, 0.8 % Agar), transferred to a new agar tube every 2 months
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data specified: Compositions of stock solutions given in the report
- Test temperature:
- 25 +/- 2°C
- pH:
- Without algae: initial: 8.0 (control), 7.9 (loading rate 100 mg/L); 72 h: 7.9 (control), 8.0 (loading rate 100 mg/L)
With algae: initial: 8.0 (control), 8.0 (loading rate 100 mg/L); 72 h: 8.6 (control), 8.7 (loading rate 100 mg/L) - Salinity:
- no data specified: Compositions of stock solutions given in the report
- Nominal and measured concentrations:
- Time [h] Control 100 mg/L loading
====================================
0 0.6 3.0 mg C/ml (TOC of WAFs)
72 2.8 2.9 mg C/mL (TOC of WAFs)
====================================
TOC-values confirmed the low water solubility of the test item.
An increase of the TOC-value could be observed in the control during 72 h. The TOC-value remained approximately stable (96.6 %) at
test loading rate of 100 mg/L. The increase of the TOC-value in the control is probably caused by organic components derived from
the metabolismof the algae. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL-Erlenmeyer
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Aeration: no
- Initial cells density: approx. 5000 cells/mL
- Control end cells density: approx. 150,000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: permanent illumination
- Light intensity and quality: approx. 10000 Lux (Gro-Lux fluorescent tubes)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: by microscopy with counting chambers at time 24 h, 48 h and 72 h.
- Chlorophyll measurement: no - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Growth curves of control and treated algae were comparable. - Reported statistics and error estimates:
- not applicable
Any other information on results incl. tables
The tables are excerpts from the original report
Table 3: Loading rate of the test item, cells/mL in the test flasks at 24, 48 and 72 h with mean value, section-by-section average specific growth rate [µ] during observation times 0-24 h, 24-48 h and 48-72 h, mean value of the average specific growth rate [µ], standard deviation and coefficient of variation Vc [%], inhibition [%] and mean value of inhibition of the average specific growth rate [%] during observation times 0-24 h, 24-48 h and 48-72 h
Loading rate [mg/L] |
Cells / mL |
Cells / mL Mean value |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific [%] |
Inhibition of Average Specific growth rate Mean value [%] |
24 h |
||||||||
Control 1 |
18000 |
20000 |
1.28 |
1.34 |
0.35 |
25.9 |
||
Control 2 |
29500 |
1.77 |
||||||
Control 3 |
16000 |
1.16 |
||||||
Control 4 |
11000 |
0.79 |
||||||
Control 5 |
21000 |
1.44 |
||||||
Control 6 |
24500 |
1.59 |
||||||
100 |
15000 |
17900* |
1.10 |
1.26 |
0.17 |
13.5 |
17.9 |
5.62 |
100 |
23000 |
1.53 |
-14.0 |
|||||
100 |
17500 |
1.25 |
6.4 |
|||||
100 |
18500 |
1.31 |
2.3 |
|||||
100 |
26000* |
- |
- |
|||||
100 |
15500 |
1.13 |
15.5 |
|||||
48 h |
||||||||
Control 1 |
78500 |
100083 |
1.47 |
1.61 |
0.16 |
9.99 |
||
Control 2 |
116500 |
1.37 |
||||||
Control 3 |
95000 |
1.78 |
||||||
Control 4 |
55500 |
1.62 |
||||||
Control 5 |
114500 |
1.70 |
||||||
Control 6 |
140500 |
1.75 |
||||||
100 |
108500 |
90000* |
1.98 |
1.62 |
0.22 |
13.8 |
-22.5 |
-0.28 |
100 |
93500 |
1.40 |
13.2 |
|||||
100 |
76000 |
1.47 |
9.06 |
|||||
100 |
91000 |
1.59 |
1.34 |
|||||
100 |
124500* |
- |
- |
|||||
100 |
81000 |
1.65 |
-2.41 |
|||||
72 h |
||||||||
Control 1 |
211500 |
245583 |
0.99 |
0.94 |
0.24 |
25.74 |
||
Control 2 |
248500 |
0.76 |
||||||
Control 3 |
244500 |
0.95 |
||||||
Control 4 |
220000 |
1.38 |
||||||
Control 5 |
261000 |
0.82 |
||||||
Control 6 |
288000 |
0.72 |
||||||
100 |
297500 |
237600* |
1.01 |
0.96 |
0.14 |
14.0 |
-7.82 |
-3.00 |
100 |
238000 |
0.93 |
0.13 |
|||||
100 |
180000 |
0.86 |
7.83 |
|||||
100 |
210500 |
0.84 |
10.4 |
|||||
100 |
547500* |
- |
- |
|||||
100 |
262000 |
1.17 |
-25.5 |
Remark: * Results of cells/mL of replicate 5 of test loading rate 100 mg/L were identified as outliers according to Grubbs Test and were excluded from calculation of the mean value of cells/mL, calculation of the growth curves, average specific growth rate and inhibition of the average specificic growth rate.
Table 4: Loading rate of the test item, cells/mL in the test flasks at 72 h, average specific growth rate [µ] over the whole testing period 0-72 h, mean value of the average specific growth rate [µ], standard deviation and coefficient of variation Vc [%], inhibition [%] and mean value of inhibition [%] of the average specific growth rate
Loading rate [mg/L] |
Cells / mL |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific growth rate [%] |
Inhibition of Average Specific growth rate Mean value [%] |
72 h |
|||||||
Control 1 |
211500 |
1.25 |
1.30 |
0.04 |
2.90 |
||
Control 2 |
248500 |
1.30 |
|||||
Control 3 |
244500 |
1.30 |
|||||
Control 4 |
220000 |
1.26 |
|||||
Control 5 |
261000 |
1.32 |
|||||
Control 6 |
288000 |
1.35 |
|||||
100 |
297500 |
1.36 |
1.28 |
0.08 |
6.55 |
-5.07 |
1.10 |
100 |
238000 |
1.29 |
0.67 |
||||
100 |
180000 |
1.19 |
7.85 |
||||
100 |
210500 |
1.25 |
3.83 |
||||
100 |
547500* |
- |
- |
||||
100 |
262000 |
1.32 |
-1.80 |
Remark: * Results of cells/mL of replicate 5 of test loading rate 100 mg/L were identified as outliers according to Grubbs Test and were excluded from calculations of the average specificic growth rate and inhibition of the average specificic growth rate.
Table 5: Loading rate of the test item, cells/mL in the test flasks at 72 h, yield over the whole test period 0 -72 h, average of yield, standard deviation and coefficient of variation Vc [%], inhibition [%] and average of inhibition of yield [%]
Loading rate [mg/L] |
Cells/mL |
Yield Cells/mL |
Yield Cells/mL Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Yield [%] |
Inhibition of Yield [%] Average |
72 h |
|||||||
Control 1 |
211500 |
206500 |
240583 |
27795 |
11.6 |
||
Control 2 |
248500 |
243500 |
|||||
Control 3 |
244500 |
239500 |
|||||
Control 4 |
220000 |
215000 |
|||||
Control 5 |
261000 |
256000 |
|||||
Control 6 |
288000 |
283000 |
|||||
100 |
297500 |
292500 |
232600 |
45375 |
19.5 |
-21.6 |
3.32 |
100 |
238000 |
233000 |
3.15 |
||||
100 |
180000 |
175000 |
27.3 |
||||
100 |
210500 |
205500 |
14.6 |
||||
100 |
547500* |
542500* |
- |
||||
100 |
262000 |
257000 |
- 6.82 |
Remark: * Results of cells/mL of replicate 5 of test loading rate 100 mg/L were identified as outliers according to Grubbs Test and were excluded from calculations of yield and inhibition of yield.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item caused no inhibition of the average specific growth rate over the whole testing period 0-72 h and during observation
intervals 0-24 h, 24-48 h and 48-72 h.
The test item caused no inhibition of yield during test period 0-72 h.
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