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EC number: 701-305-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Soil ecotoxicity of polycyclic aromatic hydrocarbons in relation to soil sorption, lipophilicity, and water solubility (§)
- Author:
- Sverdrup LE, Nielsen T, Krogh PH
- Year:
- 2 002
- Bibliographic source:
- Environ. Sci. Technol. 36, 2429-2435
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
- Principles of method if other than guideline:
- Other PAH have also been tested in this study.
- GLP compliance:
- no
- Application method:
- soil
Test material
- Reference substance name:
- Benzo[def]chrysene
- EC Number:
- 200-028-5
- EC Name:
- Benzo[def]chrysene
- Cas Number:
- 50-32-8
- Molecular formula:
- C20H12
- IUPAC Name:
- benzo[pqr]tetraphene
- Test material form:
- solid
- Details on test material:
- - Substance type: organic
Phys.-chem. properties (data from EU 2008: Coal-Tar Pitch, high temperature - Risk Assessment. European Union Risk Assessment Report, The Netherlands, and WHO 1998: EHC 202)
- Vapour pressure: 7.3E-7 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 1.5 - 3.8 µg/L (20 - 25 °C);
- Henry's law constant: 0.034 Pa*m³/mol (at 20 °C)
- log Pow: = 6.13 - 6.5
- pKa: not applicable
- Stability in water: limited due to photolytic decomposition and evaporation
- Stability in light: instable in the presence of UV
- pH dependance on stability: not applicable
Constituent 1
- Specific details on test material used for the study:
- - Name of test material as cited in study report: benzo[a)]yrene
- Source: Sigma-Aldrich, St. Louis, MO
- Purity: >98 %
- Stability in water: limited due to photolytic decomposition and evaporation
- Stability in light: instable in the presence of UV
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Soil samples of 10, 100, and 1000 mg/kg soil (dw)
Analysis at start and at the end of experiment
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of test material application: test substance dissolved in acetone was added to each container (26 g dw soil/container)
- Volume of test solution applied: 4 ml
- Controls: acetone (4ml) without test substance was applied
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): aceton
- Concentration of vehicle in test medium (stock solution and final test solution): not applicable
- Evaporation of vehicle before use: yes; evaporation in fume hood for 24 h
Test organisms
- Test organisms (species):
- Folsomia fimetaria
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- TEST ORGANISM
- Common name: springtail
- Source: synchronized animals, no further information
- Age at test initiation (mean and range, SD): 23-26 d
- Weight at test initiation (mean and range, SD): no data
Study design
- Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 20 ±1 °C
- pH (if soil or dung study):
- see details below
- Humidity:
- 50 % of water holding capacity
- Photoperiod and lighting:
- - Photoperiod: 12:12 h (light:dark)
- Light intensity: 400-800 lux - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): no data
- Amount of soil or substrate: 26 g ± 0.05 g artificial soil
- No. of organisms per container (treatment): 10 males, 10 females
- No. of replicates per treatment group: 4
- No. of replicates per vehicle control: 4
SOURCE AND PROPERTIES OF SUBSTRATE
- Artificial soil test substrate: Danish agricultural soil (Askov, Jutland)
- Composition: Sandy loam
Coarse sand (0.2 – 2 mm) 38.4 %, fine sand (0.063 – 0.2 mm) 23.6 %, coarse silt (0.02 – 0.063 mm) 10.0 %, fine silt (0.002 – 0.02 mm) 12.3 %, clay (<0.002 mm)
13 %, humus 2.8 %
- Organic carbon content: 1.6 %
- Soil pH: 6.2, no adjustment
- Density: 1.135 g/cm3,
- Cation exchange capacity: 8.14 meq/100 g
- Pretreatment of soil: samples were sieved through a 2 mm mesh before use
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h (light/dark)
- Light intensity: 400-800 lux
EFFECT PARAMETERS MEASURED: Mortality, reproduction at end of exposure time (21 d)
VEHICLE CONTROL PERFORMED: yes (details see above)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3 - 3.3
- Range finding study: no data - Nominal and measured concentrations:
- Nominal: 3, 10, 30, 100, 300, and 1000 mg/kg soil (dw)
Measured for 10, 100, and 1000 mg/kg soil (dw), recovery: 8.3, 79, and 840 mg/kg soil (dw) prior to exposure
Ratio measured / nominal concentration: 81.7 % - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 840 mg/kg soil dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: highest concentration tested
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 840 mg/kg soil dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Details on results:
- Measured concentrations of test substance after test period (21 d):
10 mg/ kg soil (dw) nominal: 5.8 mg/ kg soil (dw)
100 mg/ kg soil (dw) nominal: 66 mg/ kg soil (dw)
1000 mg/ kg soil (dw) nominal: 840 mg/ kg soil (dw)
Ratio measured/ nominal concentrations: 69.2 % - Reported statistics and error estimates:
- EC10 values (reproduction): estimated by logistic regression by SAS/STAT PROCLIN
LC50 values (mortality): estimated using probit analysis in SAS/STAT
Applicant's summary and conclusion
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