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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 02 to July 15, 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes (incl. QA statement)

Test material

1
Chemical structure
Reference substance name:
Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
EC Number:
291-716-4
EC Name:
Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
Cas Number:
90459-71-5
Molecular formula:
Not applicable - UVCB substance
IUPAC Name:
Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
Test material form:
solid
Specific details on test material used for the study:
Test item name : Octadecanoic acid, reaction products with triethylenetetramine, chloromethane-quaternized
Batch number : I1-0000196125
Internal number : 1901277-001
Manufacturing date : July 28, 2018
Expiry date : July 28, 2019
C.A.S. number : 90459-71-5
EEC number : 291-716-4
Molecular weight : UVCB substance
Empirical formula : UVCB substance
Storage : room temperature

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Remarks:
reconstituted water
Details on test solutions:
The test medium was prepared as follows:

prior to the test start, a 10.0 mg test item/L stock solution was prepared by direct weighting of 0.0050 g of test item into 500 mL of reconstituted water. The obtained solution appeared clear with particles if test item. This solution was sonicated for about five minutes and after was stirring with magnetic stirrer for about 2 hours. After stirring the stock solution appeared clear and transparent.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
A strain of Daphnia magna Straus was supplied by MicroBioTests Inc., Belgium in July 2017 (batch number: DM060417).
Since that time, the clone has been bred in the laboratories of the Test Facility under conditions identical to those of the test, with regard to temperature, light and water quality, so that no acclimation was needed prior to testing.
It was daily fed with a fixed amount of the laboratory cultured green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) and with a suspension of the yeast Saccharomyces cerevisiae.
At the test start the organisms used in the test were less than 24 hours old and were not first brood progeny.

For the test concentration and for the negative control, group of 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to the test vessels at test initiation. The daphnids loading rate was about 8 mL per one daphnid

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
140 - 250 mg/L as CaCO3
Test temperature:
19.9 – 20.1 °C.
pH:
negative control: 7.04 – 7.12
test item solution: 7.18 – 7.89
Dissolved oxygen:
negative control: 6.36 – 7.82 mg O2/L
test item solution: 6.13 – 7.39 mg O2/L
Nominal and measured concentrations:
Negative control (reconstituted water without test item) and 0.4, 0.9, 2.1, 4.6 and 10.0 mg test item/L.
Details on test conditions:
Light intensity: 1222 - 1293Lux.
Light Regime: 16 hours light and 8 hours dark.

Reference substance (positive control):
yes
Remarks:
Potassium dichromate solution

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2.2 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
0.9 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility

Any other information on results incl. tables

The observed immobilization in the negative control and test item concentration is reported in following Table.

  

Nominal test item

concentration

(mg/L)

No. of
Daphnia
tested

No. of immobilized
Daphnia

% of immobilized
Daphnia

24 h

48 h

24 h

48 h

0.0 (negative control)

20

0

0

0

0

0.4

20

0

0

0

0

0.9

20

0

11

0

55

2.1

20

8

18

40

90

4.6

20

20

20

100

100

10.0

20

20

20

100

100

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A full test at five concentrations, 0.4, 0.9, 2.1, 4.6 and 10.0 mg test item/L, was performed to evaluate the effect of the test item on Daphnia magna under static conditions.

The daphnids were observed for immobility after 24 and 48 hours of exposure.
EC0, EC100, EC50 (with their confidential limit) and NOEC, expressed in terms of nominal test item concentration, were as follows:

Time (h) EC0 (mg/L) EC100 (mg/L) EC50 (mg/L) NOEC (mg/L)
24 0.9 4.6 2.2 (1.9 – 2.7) 0.9
48 0.4 4.6 1.0 (0.8 – 1.2) 0.4


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