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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: test according to OECD 202 and GLP guideline
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
-
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,5-dihydro-3-isopropyl-8-methyl-[1,3]dioxepino[5,6-c]pyridin-9-yl acetate
EC Number:
296-417-2
EC Name:
1,5-dihydro-3-isopropyl-8-methyl-[1,3]dioxepino[5,6-c]pyridin-9-yl acetate
Cas Number:
92671-67-5
Molecular formula:
C14H19NO4
IUPAC Name:
8-methyl-3-(propan-2-yl)-1H,3H,5H-[1,3]dioxepino[5,6-c]pyridin-9-yl acetate
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
test substance not difficult

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM

- Strain: Daphnia magna Stamm Berlin
- Source: LAUS GmbH
- Age at study initiation: 0 - 23 hours
- Feeding during test: no feeding


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same as test



Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
-

Test conditions

Hardness:
no data
Test temperature:
20 +- 2°C
pH:
7.7 - 8.0
Dissolved oxygen:
8.5 - 9.3 mg/l
Salinity:
no data
Nominal and measured concentrations:
-
Details on test conditions:
TEST SYSTEM

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: potable water

OTHER TEST CONDITIONS
- Photoperiod: 16 / 8
Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
150 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Remarks on result:
other: 95% CL 108 - 300 mg/l
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: not recorded
Results with reference substance (positive control):
- Results with reference substance valid? Y

- EC50/LC50: 1.81 mg/l
- Other: 24h EC100i: 3.0 mg/l
NOEL 24h : 1.25 mg/l
Reported statistics and error estimates:
no data

Any other information on results incl. tables

no additional remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
NOEL 24 h 32 mg/l
24h EC50i 150 mg/l
24h EC100i 320 mg/l
Executive summary:

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure. In the final EC50 test Daphnia were exposed for a maximum of 24 hours to 8 different concentrations (320, 220, 150, 100, 68, 46, 32, 0 mg/l)

The study included a blank-control. The test was performed in quadruplicate with 5 daphnia

per vessel. The 24h EC50 was determined to 150 mg/l (95% range: 108 - 300 mg/l).

24 h EC50 (reference substance) = 1.81 mg/l