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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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toxicity to microorganisms, other
deactivated phrase
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
other: EEC Directive 87/302, part C
GLP compliance:
Specific details on test material used for the study:
- Name of test material (as cited in study report): Amide #71
Test type:
other: aerobic
Total exposure duration:
0.5 h
0.5 h
Dose descriptor:
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:
not specified
The IC50 (0.5 h) of 1,3-bis[12-hydroxy-octadecamide-N-methylene]-benzene for aerobic microorganisms is > 100 mg/L based on the inhibition of total respiration.
Executive summary:

The toxicity to microorganisms of 1,3-bis[12-hydroxy-octadecamide-N-methylene]-benzene is assessed in a guideline study according to EEC Directive 87/302, part C. The IC50 (0.5 h) is > 100 mg/L based on the inhibition of total respiration.

activated sludge respiration inhibition testing
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
the study does not need to be conducted because the substance is highly insoluble in water, hence indicating that aquatic toxicity is unlikely to occur

Description of key information

The substance consists of three structurally similar bisamides: N,N'-ethane-1,2-diylbis(12-hydroxyoctadecanamide), N,N'-hexane-1,6-diylbis(12-hydroxyoctadecanamide) and N,N'-[1,3-phenylenebis(methylene)]bis(12-hydroxyoctadecanamide). Experimental data on this substance are not available. The individual constituents and mixtures of them and similar amides are considered suitable read-across substances. In a guideline study on the read-across substance 1,3-bis[12-hydroxy-octadecamide-N-methylene]-benzene no significant inhibition in the respiration rate of sludge was recorded at 100 mg/litre of the test material. Based on this experimental results on the read-across substance the properties of the multi-constituent substance are expected to be comparable. Furthermore, experiments on the aquatic toxicity of the substance are considered technically not feasible due to the low water solubility and no study was conducted. The CLP criteria are not met and the substance does not need to be classified.

Key value for chemical safety assessment

Additional information

The EC50 values for individual constituents calculated by ChemProp are disregarded for assessment because the constituents were out of the applicability domains of the used models.