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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04.04.2019 - 22.07.2019
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
13 April 2004
GLP compliance:
Specific details on test material used for the study:
- Source and package No.of test material: lot #19953859
- Expiration date of the lot/batch: 16.01.2020
Analytical monitoring:
Details on sampling:
The additional test vessels for chemical analysis were prepared without daphnids in order to not disturb the chemical analysis. Due to the volume loss caused by the sampling, the volume in the additional vessels for the chemical analysis were increased. After 0 h and 48 h exposure, the additional vessels of NC, A, B, C, D and E were sampled: 4 samples of 7 mL per treatment group and time. The samples were filled into 15 mL centrifuge tubes and stored in the refrigerator (2 - 8°C).
Details on test solutions:
The test was performed with Elendt M4 medium according to OECD 202 (2004).
Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid -1 × day -1. The test organisms used for the test were 3.25 – 23.0 h old at the start of the test (required < 24h).
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
Remarks on exposure duration:
16h light, 8h dark
Test temperature:
20.3 - 21.4 °C
7.8 - 8.1
Dissolved oxygen:
8.0 -8.2 mg/L
711 microSiemens/cm
Nominal and measured concentrations:
Nominal: 25, 50, 100, 200, 400 mg/L
Details on test conditions:
The test vessels were kept in an incubator (specified temperature 18 – 22 °C, constant within ± 1 °C) with 16 h light : 8 h dark. The temperature was recorded for the test duration. The pH as well as the O 2 -concentrations were measured at the start (0 h) and at the end of the test (48 h).
Reference substance (positive control):
Potassium dichromate
Key result
48 h
Dose descriptor:
Effect conc.:
>= 105.81 - <= 105.81 mg/L
Results with reference substance (positive control):
Immobilisation of the test organisms in the control at the end of the test was 0 % and therefore ≤ 10 %.
Dissolved oxygen concentration in the control and the test item treatments at the end of the test was ≥ 8.2 mg/L and therefore ≥ 3 mg/L.
Reported statistics and error estimates:
The effect occurrence after 24h and 48h exposure was calculated. With the statistical software ToxRat Professional 3.2.1 (ToxRat Solutions GmbH, Alsdorf, Germany), EC x and LOEC/NOEC were determined.
    Measured test item concentration [mg/L]
(Lowes/No Observed) Effect Concentration  24h  48h
 ELR50 319.19 105.81
 ELR20 113.49 93.53
ELR10 66.10 87.69






The test is valid according to OECD Test Guideline 202 (13 April 2004).

Validity criteria fulfilled:
The ELR50 (48h) is 105.81 [mg/L]
Executive summary:

The Daphnia magna Acute Immobilisation Test was performed as water accommodated fraction (WAF) with five Loading Rates (LR) between 25 mg/L and 400  mg/L.  The result for ELR50 (48h) is 105.81 [mg/L]

Description of key information

Key value for chemical safety assessment

Additional information