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The ecotoxicity data from substituted amino acid (2) solution is read across to Sodium salts of substituted amino acid (2). This read across is considered justified as during ecotoxicity testing, diluted test concentrations are prepared in a matrix with sufficient buffer capacity. At higher concentrations e.g. in stock solutions the test substance is neutralized when the pH is outside the biologically optimal range. The difference between both substances is therefore considered negligible in the perspective of ecotoxicity testing.

 

For substituted amino acid (2) solution (CAS no.: CBI), which is an industrial intermediate substance, two aquatic ecotoxicity test results are available and information on the inhibition of micro-organisms presented in activated sludge and in river water.

 

Based on the results of a short-term aquatic toxicity study, performed according to OECD 201 and GLP principles, substituted amino acid (2) solution inhibited growth of Pseudokirchneriella subcapitata at analytically confirmed nominal concentrations of 18 mg/L and higher. Based on biological relevance, the 72 h-NOErC was determined to be 100 mg/L. The 72 h-ErC50 and 72h-ErC10 and NOEC were determined to be >100 mg/L.

 

Based on the results of a short-term aquatic toxicity study, performed according to OECD 202 and GLP principles, substituted amino acid (2) solution showed no toxicity towards Daphnia magna in a 48 hour exposure period when tested up to a limit concentration of 100 mg/L. Therefore, the 48h-EC50 was determined to exceed an analytically confirmed nominal concentration of 100 mg/L.

 

The toxicity of substituted amino acid (2) solution FC-C 13587 to activated sludge was determined according to OECD 209 under GLP conditions at a contact time of 3 hours, using various concentrations of the test substance. The inhibitory effect of substituted amino acid (2) solution FC-C 13587 at a particular concentration is expressed as a percentage of the two controls. From the results EC values were calculated. The EC10 of substituted amino acid (2) solution FC-C 13587 for activated sludge after 3 hours contact time is 164.3 mg a.i./L with 95% confidence limits of 76.7 and 815.4 mg/L. The EC20, EC50 and EC80 of substituted amino acid (2) solution FC-C 13587 for activated sludge could not be determined. Substituted amino acid (2) solution FC-C 13587 is therefore considered not harmful to activated sludge.

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