Registration Dossier
Registration Dossier
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EC number: 827-277-8 | CAS number: 1771689-37-2
Endpoint summary
Administrative data
Description of key information
Ready biodegradability
Key, OECD 301 F, GLP, 0.4% in 28 days: not readily biodegradable
Additional information
Ready biodegradability
The ready biodegradability test of the test item was conducted with unacclimatised sewage micro-organisms by measuring oxygen consumption over the 28-day test period according to OECD 301F and under GLP conditions.
The test item was added directly to test vessels at a level of 22.14 mg/L (equivalent to 53.35 mg/L as Theoretical Oxygen Demand or “ThODNH3”) (average, 2 replicate). The test concentration of sodium benzoate, used as reference substance in the Procedure Control, was 30.86 mg/L (equivalent to 51.54 mg/L as ThOD). In the Toxicity Control (TC) containing both test item and reference substance, the test item was added to the test vessel at a level of 22.09 mg/L(equivalent to 53.24 mg/L as ThODNH3) and the reference substance was added at a level of 31.75 mg/L of sodium benzoate (equivalent to 53.02 mg/L as ThOD). The concentration expressed as total ThOD was 106.26 mg/L. In the Abiotic Sterile Control (ASC), test vessel without inocula addition and containing sterilising agent, the test item was added to the test vessel at a level of 22.26 mg/L (equivalent to 53.65 mg/L as ThODNH3).
The biodegradation of test item after 28 days averaged 0.4% (2 replicates). The test item did not exhibit a percentage biodegradation exceeding the pass level of 60% within the 28-day period.
The biodegradation of the reference substance (Sodium benzoate) attained 63.5% after Day 3, 97.8% after Day 14 and 103.7% after Day 28.
In the Toxicity Control (TC) test mixture, 47.3% degradation occurred within 14 days and the value exceeded 25% based on total ThOD, which indicated that the test item can be assumed to be not inhibitory under the conditions of this test.
In the Abiotic Sterile Control (ASC) test vessel, 10.4% degradation occurred after 28 days.
Based on the results, the percentage biodegradation of the test item within 28 days did not exceed the pass level of 60% ThOD. The test item did not meet the criteria for “ready biodegradability” under the described test conditions.
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