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EC number: 429-780-4 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 DEC 2000 - 5 FEB 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17).The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 301C.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- 300 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- according to guideline
- Reference substance:
- aniline
- Test performance:
- The reference substance showed 77 % degradation after day 21 of the experiment and later.
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 2
- Sampling time:
- 28 d
- Details on results:
- The test substance showed 2 % degradation at day 28 of the experiment.
- Parameter:
- COD
- Value:
- 100.5 mg O2/g test mat.
- Results with reference substance:
- The reference substance showed 77 % degradation after day 21 of the experiment and later.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item was found to be NOT readily biodegradable under the described conditions.
- Executive summary:
Purpose
The purpose of this assay was to identify the biodegradation potential of the registered substance in aquatic environments to provide a rational basis for environmental fate estimation for the test item.
Study Design
The test item was investigated for its ready biodegradability in a MITI test over a period of 28 days (OECD Guideline 301C, GLP). The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Result
Biodegradation of the test material:
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period, degradation rates of 2 % and 3 % were found.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 77 % after 21 days and thereafter, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The test material was found to be not ready biodegradable under the test conditions within 28 days.
Reference
Description of key information
Key, 28 d, OECD 301C, GLP: not ready biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Purpose
The purpose of this assay was to identify the biodegradation potential of the registered substance in aquatic environments to provide a rational basis for environmental fate estimation for the test item.
Study Design
The test item was investigated for its ready biodegradability in a MITI test over a period of 28 days (OECD Guideline 301C, GLP). The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Result
Biodegradation of the test material:
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period, degradation rates of 2 % and 3 % were found.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 77 % after 21 days and thereafter, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The test material was found to be not ready biodegradable under the test conditions within 28 days.
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