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EC number: 601-420-2 | CAS number: 116265-68-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- OECD - Guideline Nc: 301 (Paris 17/07/92)
- Deviations:
- yes
- Remarks:
- Only one CO2 scrubber used per test bottle
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- 92/69/EEC C.4-C
- Deviations:
- yes
- Remarks:
- Only one CO2 scrubber used per test bottle
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]
- EC Number:
- 279-499-4
- EC Name:
- Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]
- Cas Number:
- 80584-86-7
- Molecular formula:
- C102H134O31P8
- IUPAC Name:
- Diphenyl 6,9,11,14-tetramethyl-7,13-diphenyl-4,10,16-trioxa-7,13-diphosphanonadecane-2,18-diyl bis(6,10,14-trimethyl-8,16,16-triphenoxy-4,7,9,12,15-pentaoxa-8,16-diphosphahexadecan-2-yl) bis(phosphite )
- Reference substance name:
- Triphenyl phosphite
- EC Number:
- 202-908-4
- EC Name:
- Triphenyl phosphite
- Cas Number:
- 101-02-0
- Molecular formula:
- C18H15O3P
- IUPAC Name:
- Phosphorous acid, triphenyl ester
- Reference substance name:
- Phenol
- EC Number:
- 203-632-7
- EC Name:
- Phenol
- Cas Number:
- 108-95-2
- Molecular formula:
- C6H6O
- IUPAC Name:
- phenol
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- TEST MATERIAL NAME (as stated in study report): D 25-207 (ID Code TKA 40108)
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. #06520471
- Expiration date of the lot/batch: November 1996
- Purity: > 95%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 16/01/95
- Details on inoculum:
- Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 16/01/95. The pH after collection was 7.8. The preparation was carried out according to the method described in the guideline.
- Duration of test (contact time):
- 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
- Remarks:
- Replicate 1
- Initial conc.:
- 23.9 mg/L
- Based on:
- test mat.
- Remarks:
- Replicate 2
- Initial conc.:
- 15.9 mg/L
- Based on:
- other: ThOC
- Remarks:
- Replicate 1
- Initial conc.:
- 15.2 mg/L
- Based on:
- other: ThOC
- Remarks:
- Replicate 2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2'C
- Aeration of dilution water: ~ 25 mL/min purified from carbon dioxide.
SAMPLING
- Sampling frequency: days 0, 3, 6, 8,10,13,17, 20, 24, 28 and 29.
CALCULATIONS: The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the quantities of inorganic carbon
determined on the days of measurement in the absorbers.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 15 mg DOC/L
Results and discussion
- Preliminary study:
- In mixtures with the reference substance, the test substance did not inhibit the biodegradation of the reference substance.
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 29 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In a guideline (OECD 301B) study, D 25-207 was not readily biodegradable. 0% degradation was achieved after 29 days.
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