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EC number: 948-916-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3-6 September 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Uncritical deviations: experiment temperature range 20.7 – 22.2 °C, pre-culture incubation range 19.0 – 23.3 °C, autoclaved for 20 instead of 15 min, pre-culture preparation 3-4 days before test start
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- as above
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Molecular formula:
- C51H92O6 to C69H128O6
- IUPAC Name:
- vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Reference substance name:
- dimers of vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Molecular formula:
- C102H186O12 to C138H256O12
- IUPAC Name:
- dimers of vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Reference substance name:
- trimers of vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Molecular formula:
- C153H280O18 to C207H388O18
- IUPAC Name:
- trimers of vegetable oil mono- and polyunsaturated C16-C22 triglycerides
- Reference substance name:
- tetramers and higher oligomers of vegetable oil mono- and polyunsaturated C16-C22
- Molecular formula:
- C204H374O24 and higher oligomers
- IUPAC Name:
- tetramers and higher oligomers of vegetable oil mono- and polyunsaturated C16-C22
- Test material form:
- liquid: viscous
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: The content of the test item was estimated in the test solutions at the start and the end (72 h) of the test
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The water-accommodated fractions (WAF) were prepared for the test. This was done by mixing the real loads of 1 / 3.2 / 10 / 32 / 100 mg/L resp. 1.1 / 3.5 / 10.9 / 34.8 / 108.7 µL/L test item (based on a density of 0.92 g/mL stated in the SDS) with the corresponding amount of algal medium (demineralised water enriched with minerals but without algae) and stirring moderate for 24 hours at 120 rpm on a magnetic stirrer. No turbidity of the test solution could be observed. The lower phase was used unfiltered as test solutions.
- Controls: Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate reference test.The values lay within the range of the laboratory (growth rate 0.65 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular freshwater green alga
- Strain: 86.81
- Source: The algae culture was obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen).
- Method of cultivation: The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared.
For the pre-culture an aliquot of the permanent culture was brought into nutrient medium and incubated under continuous lighting for 96 hours under test conditions (Lighting: within the specified range of 4440 – 8880 lux; 19.0 – 23.3°C; see chapter 13). The result-ing culture grew exponentially. Before usage, the pre-culture was checked for the absence of cell aggregates and the cell number of culture was determined.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- not applicable
Test conditions
- Hardness:
- no data
- Test temperature:
- 20.7 – 22.2°C
- pH:
- 7.6-8.6
- Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Conductivity:
- no data
- Nominal and measured concentrations:
- 1 / 3.2 / 10 / 32 / 100 mg/L loading rate
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks total volume 65 mL
- Material, size, headspace, fill volume: The test vessels were filled with 45 ± 1 mL
- Initial cells density: 2500 cells/mL
- Control end cells density: 531507 Cell Number/mL
- No. of vessels per concentration (replicates): 3 replicates for each treatment
- No. of vessels per control (replicates): 6 replicates for the blank control
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Light intensity and quality: within the specified range (4440 – 8880 Lux)
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: electronic particle counter every 24h, observation of cell abnormalities
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Results used to determine the conditions for the definitive study: The concentrations to be tested are based on non GLP pre-tests - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) in a separate reference test
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): microscopical observations confirmed the normal and healthy appearance of the algae
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The concentrations measured in the test solutions are in the same very low range as the blank value. It is detectable but not quantifiable. In addition, the test solutions were in direct contact with the test item during stirring. This means that the soluble components must have dissolved. Therefore, the results are related to the loading rate - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 72h ErC50 0.66 mg/L (95% C.I. 0.64 – 0.69 mg/L); 72h EyC50 0.40 mg/L (95% C.I. 0.38 – 0.41 mg/L). The values lay within the range of the laboratory (growth rate 0.65 - 1.10 mg/L, yield 0.21 – 0.66 mg/L). - Reported statistics and error estimates:
- As no inhibition could be observed no statistical evaluation was performed
Any other information on results incl. tables
A slight inhibition of algal growth was observed only in the lowest concentration (1 mg/L). However, this is probably due to contamination or carry-over, as all higher concentrated treatments do not show any inhibition.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure to test item of Desmodesmus subspicatus for 72 h did not induced any inhibition, determining an NOELR and EL50 for growth and yield greater than 100 mg/L (loading rate).
- Executive summary:
A study was performed in a Water Accommodated Fraction (WAF) test setup exposing the algae,Desmodesmus subspicatus, for 72 h to concentration range of 1.0 to 100 mg/L (loading rate) of the test item. Test was performed according to the OECD guideline 201 and it fulfilled the validity criteria. Measured concentrations were estimated by by determination of the total organic carbon (TOC) content in the test solutions using a carbon analyser. The concentrations measured in the test solutions are in the same very low range as the blank value. It is detectable but not quantifiable. Therefore, the results are related to the loading rates; see Guidance Doc. No.23 (§153). No inhibition was observed after 72 h, determining a NOELR, LOELR, EL10 and EL50 for growth and yield greater than 100 mg/L (loading rate).
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