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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Remarks:
This test was performed for the evaluation of the substance as a cosmetic ingredient and not for REACH purposes.

Test material

Constituent 1
Reference substance name:
1-(1-oxododecyl)-L-proline
Cas Number:
58725-39-6
Molecular formula:
C17H31NO3
IUPAC Name:
1-(1-oxododecyl)-L-proline
Constituent 2
Reference substance name:
1-(1-oxotetradecyl)-L-Proline
Cas Number:
86282-96-4
Molecular formula:
C19H35NO3
IUPAC Name:
1-(1-oxotetradecyl)-L-Proline
Constituent 3
Reference substance name:
1-(1-oxodecyl)-L-Proline
Cas Number:
86282-95-3
Molecular formula:
C15H27NO3
IUPAC Name:
1-(1-oxodecyl)-L-Proline
Constituent 4
Reference substance name:
1-(1-oxooctyl)-L-Proline
Cas Number:
86282-94-2
Molecular formula:
C13H23NO3
IUPAC Name:
1-(1-oxooctyl)-L-Proline
Constituent 5
Reference substance name:
1(1-oxohexadecyl)-L-proline
Cas Number:
59441-32-6
Molecular formula:
C21H39NO3
IUPAC Name:
1(1-oxohexadecyl)-L-proline
Constituent 6
Reference substance name:
Dodecanoylprolyl-Lproline
IUPAC Name:
Dodecanoylprolyl-Lproline
Constituent 7
Reference substance name:
Tetradecanoylprolyl-L-proline
IUPAC Name:
Tetradecanoylprolyl-L-proline
Constituent 8
Reference substance name:
Palmitoylprolyl-L-proline
IUPAC Name:
Palmitoylprolyl-L-proline
Constituent 9
Reference substance name:
1-(1-oxooctadecyl)-L-proline
Molecular formula:
C23H43NO3
IUPAC Name:
1-(1-oxooctadecyl)-L-proline
Constituent 10
Reference substance name:
potassium;chloride
Cas Number:
7447-40-7
Molecular formula:
KCl
IUPAC Name:
potassium;chloride
Constituent 11
Reference substance name:
Reference substance 001
Cas Number:
1310-58-3
Molecular formula:
KOH
Constituent 12
Reference substance name:
Tripotassium phosphate
Cas Number:
7778-53-2
IUPAC Name:
Tripotassium phosphate
impurity 1
Reference substance name:
Unknown impurities
IUPAC Name:
Unknown impurities
Specific details on test material used for the study:
Cocoyl proline is produced directly in water solution. In this test, cocoyl proline was tested in the commercialized form, so in aqueous solution.

This choice was taken considering that for measuring toxicity on fishes, solids need to be dissolved in water before testing. Furthermore, it provides a better understanding on the properties of the substance in the form that will be in contact with final users

Water percentage in the sample varies between 30 and 50%.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Total exposure duration:
72 h

Test conditions

Test temperature:
23°C ± 1°C

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
0.03 g/L
Details on results:
The test was performed at the following concentrations:
1 g/L, 0.5 g/L, 0.25 g/L, 0.125 g/L, 0.06 g/L, 0.01 g/L

All the fish died (7 specimen for each concentration) for the tests up to 0.06 g/L. No animal died in the test with 0.01 g/L of test substance.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Cocoyl Proline was tested for fish toxicity, with the final result of 0.03 g/L of LC50 for Danio Rerio.

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