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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
The effect concentration was measured after 24h.
GLP compliance:
not specified
Remarks:
This test was performed for the evaluation of the substance as a cosmetic ingredient and not for REACH purposes.

Test material

Constituent 1
Reference substance name:
1-(1-oxododecyl)-L-proline
Cas Number:
58725-39-6
Molecular formula:
C17H31NO3
IUPAC Name:
1-(1-oxododecyl)-L-proline
Constituent 2
Reference substance name:
1-(1-oxotetradecyl)-L-Proline
Cas Number:
86282-96-4
Molecular formula:
C19H35NO3
IUPAC Name:
1-(1-oxotetradecyl)-L-Proline
Constituent 3
Reference substance name:
1-(1-oxodecyl)-L-Proline
Cas Number:
86282-95-3
Molecular formula:
C15H27NO3
IUPAC Name:
1-(1-oxodecyl)-L-Proline
Constituent 4
Reference substance name:
1-(1-oxooctyl)-L-Proline
Cas Number:
86282-94-2
Molecular formula:
C13H23NO3
IUPAC Name:
1-(1-oxooctyl)-L-Proline
Constituent 5
Reference substance name:
1(1-oxohexadecyl)-L-proline
Cas Number:
59441-32-6
Molecular formula:
C21H39NO3
IUPAC Name:
1(1-oxohexadecyl)-L-proline
Constituent 6
Reference substance name:
Dodecanoylprolyl-Lproline
IUPAC Name:
Dodecanoylprolyl-Lproline
Constituent 7
Reference substance name:
Tetradecanoylprolyl-L-proline
IUPAC Name:
Tetradecanoylprolyl-L-proline
Constituent 8
Reference substance name:
Palmitoylprolyl-L-proline
IUPAC Name:
Palmitoylprolyl-L-proline
Constituent 9
Reference substance name:
1-(1-oxooctadecyl)-L-proline
Molecular formula:
C23H43NO3
IUPAC Name:
1-(1-oxooctadecyl)-L-proline
Constituent 10
Reference substance name:
potassium;chloride
Cas Number:
7447-40-7
Molecular formula:
KCl
IUPAC Name:
potassium;chloride
Constituent 11
Reference substance name:
Reference substance 001
Cas Number:
1310-58-3
Molecular formula:
KOH
Constituent 12
Reference substance name:
Tripotassium phosphate
Cas Number:
7778-53-2
IUPAC Name:
Tripotassium phosphate
impurity 1
Reference substance name:
Unknown impurities
IUPAC Name:
Unknown impurities
Specific details on test material used for the study:
Cocoyl proline is produced directly in water solution. In this test, cocoyl proline was tested in the commercialized form, so in aqueous solution.

This choice was taken considering that for measuring toxicity on aquatic invertebrates, solids need to be dissolved in water before testing. Furthermore, it provides a better understanding on the properties of the substance in the form that will be in contact with final users

Water percentage in the sample varies between 30 and 50%.

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
A stock solution has been prepared dissolving a known amount of the sample in a specified volume of
dilution water (1 g/L). The test has been carried out starting from the concentration of 100 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The method requires Daphnia magna grown in laboratory under controlled conditions, while organisms (Ephippia) from specialized suppliers have been used in immobilized conditions and let open (about 72-80 hours, 6000 lux, 20-22°C, about 72-80) for the execution of the test. The crustaceans used for the test were not more than 24 hours old.

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
24 h

Test conditions

Hardness:
250 mg/L +/- 25 mg/L (on CaCO3)
Test temperature:
20-22°C
pH:
7,8 +/- 0,2
Dissolved oxygen:
7 mg/L
Conductivity:
10 μS/cm maximum
Nominal and measured concentrations:
A stock solution has been prepared dissolving a known amount of the sample in a specified volume of dilution water (1 g/L). The test has been carried out starting from the concentration of 100 mg/l. These were nominal concentrations.

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
39.3 mg/L
Basis for effect:
mobility

Applicant's summary and conclusion

Conclusions:
On the basis of the assay, cocoyl proline showed effects on motility for aquatic invertebrates, in particular the endpoint was assessed at 39,3 mg/L (EC50 after 24h).

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