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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Die akute und chronische Toxizität der Ameisensäure und ihrer Formiate)
Author:
G. MALORNY
Year:
1969
Bibliographic source:
ZERNAL Zeitschrift fuer Ernaehrungswissenschaft. (Dr. Dietrich Steinkopff Verlag, Postfach 111008, D-6100 Darmstadt 11, Fed. Rep. Ger.) V.1- 1960- Volume(issue)/page/year: 9,332,1969

Materials and methods

Test guideline
Guideline:
other: Study conducted prior to implementation of GLP and OECD guidelines
Principles of method if other than guideline:
Study conducted prior to implementation of GLP and OECD guidelines
GLP compliance:
no
Remarks:
data published prior to implementation of GLP
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium formate
EC Number:
208-753-9
EC Name:
Ammonium formate
Cas Number:
540-69-2
Molecular formula:
CH2O2.H3N
IUPAC Name:
ammonium formate
Test material form:
solid: crystalline

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
oral application of doses up to the range of 2050- 2460mg/kg bw where LD50 values were determined.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Doses:
up to 2050 - 2460 mg/ kg bw
No. of animals per sex per dose:
50
Control animals:
not specified

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
2 250 mg/kg bw
Based on:
test mat.
Mortality:
50 % Mortality was observed
Clinical signs:
other: Details of toxic effects not reported other than lethal dose value

Any other information on results incl. tables

Beside the determination of the LD50 of ammonium formate the LD50 of fomate ion was calculated to be 1610 mg/kg bw

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
According to the study of Malorny (1969) the LD50 of ammonium formate was determined in mice at a dose of 2250 mg/kg bw after oral administration. No other signs of toxicity were reported.
Based on the Globally Harmonized System for Classification and Labelling of Chemicals (GHS) Ammonium formate has to be categorized as Category 5 "May be harmful if swalloed", according to Regulation (EC) No 1272/2008 Ammonium formate does not need to be classified for acute oral toxicity.
Executive summary:

In an acute oral toxicity study conducted prior to implementation of OECD and GLP Guidelines but comparable to OECD guideline 401, in sum 50 mice were given a single oral dose of Ammonium formate by gavage at doses up to 2050 - 2460mg/kg bw. No other adverse effects were described.

Oral LD50 (mice) =2250  mg/kg bw

Based on the Globally Harmonized System for Classification and Labelling of Chemicals (GHS) Ammonium formate has to be categorized as Category 5 "May be harmful if swalloed", according to Regulation (EC) No 1272/2008 Ammonium formate does not need to be classified for acute oral toxicity.