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Toxicity to aquatic algae and cyanobacteria

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Description of key information

Under the conditions of the test, no effects were observed at saturation of the hydrolysis product. 72 hour ErC50 > 100 % v/v (saturated solution), NOEC(growth rate) = 100 % v/v (saturated solution), OECD 201, EU Method C.3, Vryenhoef & Mullee 2011.

Key value for chemical safety assessment

Additional information

The acute toxicity of the test material to the algae was determined using Pseudokirchnerella subcapitata in a study performed under GLP conditions and in accordance with the standardised guidelines OECD 201 and EU Method C.3.

Since the test material is known to rapidly hydrolyse in water (half-life approximately 1-Hour in deionized reverse osmosis water) the test was conducted using the hydrolysis product in a limit test at 100 % v/v saturated solution in accordance with the OECD guidance 23 (Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures). Analysis of the test preparations was performed using HPLC-MS at 0 and 72 hour. Measured concentrations were less than the limit of quantitation (0.11 mg/L). Given that measured concentrations could not be obtained in this study the results were based on nominal concentrations.

Six replicates each containing the algal cultures at an initial cell density of 4.57 x 10^5 cells per mL were exposed the hydrolysis product of the test material in a 100 % v/v saturated solution and a blank control for 72 hours. Cultures were observed effects on growth rate and cell number.

Under the conditions of the test, no effects were observed at saturation of the hydrolysis product. Therefore the ErC50 was determined to be > 100 % v/v saturation solutions and the NOEC 100 % v/v saturation solution.This study showed that there were no toxic effects at the limit of water solubility.

This key study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 in accordance with the criteria outlined by Klimisch (1997) and considered suitable for assessment as an accurate reflection of the test material.