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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Remarks:
water
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
2 x 2 L with 100 mg/L were shaken with 20 rpm for 24 h and transferred to the gals aquarium.
- Controls:
dilution water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): n.a.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
the test item sedimented and showed turbidity throughout the test

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Strain: Danio rerio
- Source:
a. Origin of parental fish: Aquarium Am Aegi, Hannover, Germany
b. fish used for the study: in house breed Dr. Noack Laboratorium, Sarstedt, Germany
- Length at study initiation (mean): 2.02 cm
- Weight at study initiation (mean): 0.07 g
- Method of breeding/maintenance:
Holding: was performed at the test facility at 23 +/- 2°C and diffuse light (0.1-10 µmol/m2s, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80% of the air saturation value. Only fish from a population with mortality < 5% within the last 7 days before start of the study were used in the test. No disease treatments were administered throughout holding and testing.
Water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
Feeding: Food was provided 3 times per week. The amount of food was 4% of the fish body weight per feeding.


ACCLIMATION
none

FEEDING DURING TEST
The test fish were not fed until 24 h before the test started
Fish were not fed during the test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
63 mg/L as CaCO3
Test temperature:
mean of 4 days: 21.6°C
pH:
mean of 4 days: 7.46
Dissolved oxygen:
mean of 4 days: 99%
Nominal and measured concentrations:
nominal: 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel/Type:
Glass-aquaria loosely covered by glass tops
- fill volume:
approx. 4 L
- Aeration:
Gentle aeration provided
- Type of flow-through (e.g. peristaltic or proportional diluter):
static
- Renewal rate of test solution (frequency/flow rate):
one applicatin of test item at test initiation
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin, filtered on activated charcoal and aerated for at least 24 h to remove chlorine
- Intervals of water quality measurement: every 24 h

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: natural photoperiod
- Light intensity: 0.1 - 10 µE/m2 x s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
every 24 h: mortality, behaviour

TEST CONCENTRATIONS
- Range finding study : no mortality at 100 mg/L (nominal)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The test item sedimented and showed turbidity throughout the test. The nominal test item concentration of 100 mg/L caused no mortality or non-lethal effects.
The study met the validity criteria of the guideline:
- O2-saturation was >/= 60%
mortality in the control was 0%
Results with reference substance (positive control):
n.a.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this study the test substance did not cause any effects to zebrafish after 96 h when tested with a nominal concentration of 100 mg/L. The NOEC (0-96 h) is laid down as 100 mg/L.
Executive summary:

The acute toxicity of the test substance to fish (zebrafrish) was determined according to OECD guideline 203.

A static limit test with a single test item concentration of 100 mg/L was conducted. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and control. Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72, and 96 h were determined to be within the acceptable limits. All effect values are based on the nominal concentration of the test item. No analysis was performed. Due to insolubility of the test item no specific analytical method was available.

The acute toxicity of the test substance is summarised as:

LC50 (96 h) > 100 mg/L

LC100 (96 h) > 100 mg/L

LC0 (96 h): 100 mg/L

NOEC (96 h): 100 mg/L