4-trans-Methoxy-4-trans-propyl-[1,1-bicyclohexyl]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2010-09-13 to 2010-11-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Use of solvents to increase exposure; Pre test for bioaccumulation study. This study was performed according to GLP and the methods applied are fully compliant with OECD TG 203.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

0; 24; 48; 72; 96 h

Vehicle:

yes

Details on test solutions:

For preparation of the test solution, Tween 80 was used as solubilizer. The concentration of the vehicle was 500 mg/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Lot No: 100812
Weight: 0.21 g
Length: 3.0 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Test temperature:

25.1 +/- 1.0 °C

pH:

According to guideline

Dissolved oxygen:

According to guideline

Salinity:

According to guideline

Nominal and measured concentrations:

nominal: 1.0, 1.5, 2.2, 3.3, 5.0 mg/L
measured: below limit of quantification

Details on test conditions:

10 fish per 4 L

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality control: 0 % contanining the vehicle at any timepoint
Tween 80: 0 % at any timepoint
5.0 mg/L: 100 % (24h), 100% (48h), 100% (72h), 100% (96h)
3.3 mg/L: 100 % (24h), 100% (48h), 100% (72h), 100% (96h)
2.2 mg/L: 0 % (24h), 10% (48h), 30% (72h), 40% (96h)
1.5 mg/L: 0 % (24h), 0% (48h), 0% (72h), 0% (96h)
1.0 mg/L: 0 % (24h), 0% (48h), 0% (72h), 0% (96h)
- Effect concentrations exceeding solubility of substance in test medium: All concentrations tested were at or above the solubility limit of the pure test substance. To achive the exposure concentrations, solvent Tween 80 was used.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96h) of the test material with solubilizer was 2.2 mg/L, which is clearly above the limit of water solubility.

Executive summary:

The acute toxicity of the test material was studied in a pretest for a bioaccumulation study (OECD 305) using solvents to increase exposure. The GLP test was performed according to the METI Guideline: Concentration Test on Chemical Substances in Fish. The pretest was performed according to OECD TG 203. To achieve the exposure concentrations, solvent Tween 80 was used. All concentrations tested were at or above the solubility limit of the pure test substance. For the test Brachydanio rerio (new name: Danio rerio) was used. The following nominal concentrations have been applied: 1.0, 1.5, 2.2, 3.3, 5.0 mg/L. At 5 and 3.3 mg/L, 100 % mortality was observed at any time point. The EC50 (96h) was at 2.2 mg/L using Tween 80 as solubilizer. All validity criteria were met. The test material does not possess acute toxic potential to fish up to the limit of solubility in water. The LC₅₀ (96h) of the test material with solubilizer was 2.2 mg/L, which is clearly above the limit of water solubility.

Description of key information

The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203. The LC₅₀ (96h) of the test material with solubilizer was 2.2 mg/L, which is clearly above the limit of water solubility.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2.2 mg/L

Additional information

The acute toxicity of the test material was studied in a pretest for a bioaccumulation study (OECD 305) using solvents to increase exposure. The GLP test was performed according to the METI Guideline: Concentration Test on Chemical Substances in Fish. The pretest was performed according to OECD TG 203. To achieve the exposure concentrations, solvent Tween 80 was used. All concentrations tested were at or above the solubility limit of the pure test substance. For the test Brachydanio rerio (new name: Danio rerio) was used. The following nominal concentrations have been applied: 1.0, 1.5, 2.2, 3.3, 5.0 mg/L. At 5 and 3.3 mg/L, 100 % mortality was observed at any time point. The EC50 (96h) was at 2.2 mg/L using Tween 80 as solubilizer. All validity criteria were met. The test material does not possess acute toxic potential to fish up to the limit of solubility in water. The LC₅₀ (96h) of the test material with solubilizer was 2.2 mg/L, which is clearly above the limit of water solubility.