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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 May, 2015 to 07 October, 2015
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
July, 1992
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
NYMPHEAL (GR-88-0778) :
Lot No. : Batch 6
Purity : 99.1% (sum of isomers)
Date of Expiry : 29 March, 2017
Analytical monitoring:
yes
Remarks:
GC-MS
Details on sampling:
Water samples were taken, extracted and analysed at the Following time-points for exposure concentrations C1 to C5 :
- Fresh exposure medium : 0 hrs and at all subsequent renewals (24hrs, 48hrs, 72hrs);
- Aged exposure medium : 24 hrs, 48 hrs, 72 hrs and 96 hrs
Samples of the Control and the Solvent Control were sampled, extracted and analysed at : Time 0 (fresh) and aged medium at 96 hours.
Vehicle:
yes
Remarks:
Acetone, at maximum concentration of 0.1 mL/L.
Details on test solutions:
Following a Range-Finding study, the Following Nominal test concentrations were chosen for the definitive study :
C1 0.48 mg/L
C2 0.86 mg/L
C3 1.54 mg/L
C4 2.78 mg/L
C5 5.00 mg/L

The exposure solutions were prepared using standards prepared in Acetone as a vehicle. Since NYMPHEAL is a primary aldehyde, and, therefore, susceptible to undergo rapid oxidation, it was decided to avoid long, drawn-out exposure medium preparation techniques (e.g. slow-stirring to obtain water saturated fraction).

A blank Control and Solvent Control were also performed.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Danio rerio in-house culture
Spawning Date : Nov. 2014
Length of Fish : 2.63 cm (representative group of 10 fish, 3 days prior to beginning of exposure)
Body Weight of Fish : 0.164 g/fish (representative group of 10 fish, 3 days prior to beginning of exposure)
Temperature of Culture : 24.7 - 24.9 °C
Mortalities during period 7-days prior to Start of Study : 0%
Food : TetraMin, ad libitum
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
254 - 264 mg/L of CaCO3
Test temperature:
21.1 to 23.9 °C
pH:
pH 7.7
Dissolved oxygen:
7.5 - 8.5 mg O2/L
Conductivity:
692 microS/cm2
Nominal and measured concentrations:
The water samples were simultaneously analysed for the residue of the Parent, and the corresponding alcohol (GR-88-1108) and acid (GR-88-1910).

NYMPHEAL was not stable in the exposure solutions over the 24-hour renewal period. The concentrations determined in the Fresh medium samples varied in the range of 68 to 103 % of Nominal, and, 33 - 86% of Nominal in the 24 hour old medium.

The Time-Weighted Mean Measured concentrations of the Parent over the 96-hour exposure period were, as follows :

Exposure Level Time-Weighted Mean Concn.
Control Not Detected
Solvent Control Not Detected
C1 (0.48 mg/L) 0.293 mg/L
C2 (0.86 mg/L) 0.489 mg/L
C3 (1.54 mg/L) 0.986 mg/L
C4 2.78 mg/L) 2.29 mg/L
C5 (5.00 mg/L) 4.34 mg/L

Small amounts of the Acid Metabolite were detected in the 24 hour aged samples, and, higher quantities of the Alcohol metabolite. Although total equivalent concentrations ahve been reported in the Final report, the end-points reported hereinare related to Parent, only.
Details on test conditions:
Exposure Vessels : Fish exposed in 4 L glass tanks, depth of test solutio 15.2 cm, 7 fish per tank;
Fish Loading : 0.287 g/L
Length of Fish : 2.631 cm
Average Body Weight : 0.164 g/fish
Renewal : Every 24-hours at 24, 48 and 72 hours
Feeding : None
Photoperiod : Light/Dark 12h/12h
Light Intensity : 457 - 980 Lux
Aeration : Gentle aeration (permanent)
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.09 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% Confidence Interval : 0.546 - 1.72 mg/L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.489 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
other:
Remarks:
Absence of any sub-lethal observed effects.
Details on results:
A dose-response Relationship was observed.
Validity criteria fulfilled:
yes
Conclusions:
The 96 hour LC50 observed for D. rerio exposed to NYMPHEAL is 1.09 mg/L.
Executive summary:

The 96 hour LC50 observed for D. rerio exposed to NYMPHEAL is 1.09 mg/L

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Description of key information

OECD 203 - Acute Fish Toxicity :


LC50 (96 hr) (Danio rerio) = 1.09 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.09 mg/L

Additional information

OECD 203 - Acute Fish Toxicity :


LC50 (96 hr) (Danio rerio) = 1.09 mg/L

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