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Reaction mass of disodium 4-amino-6-[[4-[(2,4-diaminophenyl)diazenyl]phenyl]]-3-((E)-[4-((E)-(2,4-diaminophenyl)diazenyl]phenyl )diazenyl)-5-hydroxynaphthalene-2,7-disulphonate and disodium 4-amino-6-((E)-(4-aminophenyl)diazenyl)-3-((E)-(4-((E)-(2,4-diaminophenyl)diazenyl)phenyl)diazenyl)-5-hydroxynaphthalene-2,7-disulfonate
EC number: 951-695-0 | CAS number: -
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: experimetal study on similar substance
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The test was performed following official guideline, some details on test conditions are missing. Study meets generally accepted scientific principles,acceptable for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 7340
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 4-amino-3,6-bis[[4-[(2,4-diaminophenyl)azo]phenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
- EC Number:
- 229-208-1
- EC Name:
- Disodium 4-amino-3,6-bis[[4-[(2,4-diaminophenyl)azo]phenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
- Cas Number:
- 6428-31-5
- Molecular formula:
- C34H29N13O7S2.2Na
- IUPAC Name:
- disodium 4-amino-3,6-bis({4-[(2,4-diaminophenyl)diazenyl]phenyl}diazenyl)-5-hydroxynaphthalene-2,7-disulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Poecilia reticulata
Study design
- Test type:
- not specified
- Water media type:
- freshwater
Test conditions
- Nominal and measured concentrations:
- The stock solutions of the test substance of nominal concentration 1 g/l was prepared. The concentrations of solutions used in the basic test were obtained by dilution of the stock solution with dilution water. Dilution used for basic test : 1000 ml/l, 900 ml/l, 700 ml/l and 500 ml/l.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results show no toxicity on fish.
- Executive summary:
The test performed on the tested items follows the ISO 7340 and shows no toxicity on the test organisms.
LC50 is > 1000 mg/l.
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