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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998 - 1990
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Reference substance name:
4-mercaptomethy1-3,6-dithia-1,8-octanedithiol
IUPAC Name:
4-mercaptomethy1-3,6-dithia-1,8-octanedithiol
Test material form:
solid - liquid: suspension
Details on test material:
- Lot/batch No.: 111

Test animals

Species:
rat
Strain:
Crj: CD(SD)
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Doses:
250, 500, 1000, 2500, 4000 and 5000 mg/kg
No. of animals per sex per dose:
Range-finding Study: 1 animal/sex/dose.
LD50-Determination: 5 animals/sex/dose.
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
3 428 mg/kg bw
Based on:
test mat.
95% CL:
2 354 - 4 994
Sex:
male
Dose descriptor:
LD50
Effect level:
3 643 mg/kg bw
Based on:
test mat.
95% CL:
1 779 - 7 458
Sex:
male/female
Dose descriptor:
LD50
Effect level:
3 577 mg/kg bw
Based on:
test mat.
95% CL:
2 651 - 4 826
Mortality:
Male: 1200 mg/kg bw/d; Number of animals: 5; Number of deaths: 0
Male: 2500 mg/kg bw/d; Number of animals: 5; Number of deaths: 2
Male: 5000 mg/kg bw/d; Number of animals: 5; Number of deaths: 3
Female: 1200 mg/kg bw; Number of animals: 5; Number of deaths: 0
Female: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 1
Female: 5000 mg/kg bw; Number of animals: 5; Number of deaths: 4
Clinical signs:
Signs seen on the day of dosing in most groups included oral discharge, urinary and fecal staining, soft stool and hypoactivity. Additional signs seen on the day after dosing included nasal discharge and abdominal griping in the 5000 mg/kg dose group only. On the day after dosing, ataxia, convulsions, coarse tremors, hypopnea, irregular breathing and abdominal griping were seen in the 2500 mg/kg dose group. All surviving animals had decreased food consumption on the day after dosing; this continued in some animals through Day 7.
Body weight:
All surviving animals in the 1200 and 2500 mg/kg dose groups gained weight both 7 and 14 days after dosing. All surviving animals in the 5000 mg/kg dose group lost weight at Day 7, but gained weight between Days 7 and 14.
Gross pathology:
Postmortem examinations of animals which were found dead revealed a variety of changes, primarily in the lungs and gastrointestinal tract. Some animals which were found dead exhibited changes in the stomach and intestine which were suggestive of an irritant and/or corrosive effect (discoloration of walls, the presence of red, tan or black material). Other changes in animals found dead appeared to represent autolytic changes or were the result of ante-mortem stress (testes in the body cavity). Changes in animals killed after 14 days were similar to those seen in control animals in this laboratory killed by carbon dioxide inhalation or were considered to represent normal physiological variation.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The oral toxicity of DMPT in rats is low (LD50 above 3,000 mg/kg/d). The anticipated mean oral LD50 of 3577 mg/kg/d of DMPT allows classification in OECD GHS Toxicity Category IV.