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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Zinc cyanide
EC Number:
209-162-9
EC Name:
Zinc cyanide
Cas Number:
557-21-1
Molecular formula:
Zn(CN)2
IUPAC Name:
zinc(2+) bis(iminomethanide)
Test material form:
solid: particulate/powder

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
ENVIRONMENTAL CONDITIONS: the animal room environment was automatically controlled according to KIT SOPs(target range: temperature 20±3°C, relative humidity 30-70%, approximatly 12 hours light/ 12 hours dark cycle with 150-300 Lux, and ventilation 10-20 times/hour). Temperature and relative humidity were monitored continuously. Animal room and cage cleaning was performed according to KIT SOPs. Cage card and animal room use record were attached on the cage and the entrance of the animal room, respectively.

Test system

Type of coverage:
not specified
Preparation of test site:
shaved
Vehicle:
not specified
Amount / concentration applied:
0.5g
Observation period:
1, 24, 48 and 72 hours
Number of animals:
3

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Time point:
24/48/72 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Time point:
24/48/72 h
Score:
0
Max. score:
0
Irritant / corrosive response data:
No dermal irritation was observed at 1, 24, 48, 72 hours after test item application
Other effects:
Test item-related death or abnormal general clinical signs were not observed during the study period.
Test item-related body weight changes were not observed.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
To investigate dermal irritation of the test item, Zinc cyanide was administered by dermal at dose of 0.5 g/head for 4 hours in 3 male New Zealand White rabbits. Mortality, clinical signs, body weight change and local irritation were observed until 72 hours after test item. No clinical sign, body weight, dead animaln or abnormal skin reaction considered to be due to test item were observed. In conclusion Zinc cyanide induced no dermal irritaiton in male New Zealand White rabbits and was considered to be a non-irritant.
Executive summary:

For that endpoint, one reliable study to assess skin irritation and corrosion in vivo on the registered substance on New Zealand White rabbits was available. The study was performed according to the OECD Guideline 404 (Acute Dermal Irritation / Corrosion).

Under the experimental conditions, no skin reaction was observed with "Zinc cyanide batch n°10185145 " exposure. Therefore, "Zinc cyanide batch n°10185145" induced no dermal irritaiton in male New Zealand White rabbits and was considered to be a non-irritant.

The validity criteria were successful and the study was therefore regarded as acceptable for that endpoint.