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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Zinc cyanide
EC Number:
209-162-9
EC Name:
Zinc cyanide
Cas Number:
557-21-1
Molecular formula:
Zn(CN)2
IUPAC Name:
zinc(2+) bis(iminomethanide)
Test material form:
solid: particulate/powder

Test animals / tissue source

Species:
rabbit
Strain:
other: Yac:NZW(KBL)
Details on test animals or tissues and environmental conditions:
The animal room environment was automatically controlled according to KIT SOPs (target range: temperature 20+/- 3°C, relative humidity 30-70%, approximately 12 hours light/12 hours dark cycle with 150-300 Lux, and ventilation 10-20 times/hour). Temperature and relative humidity were monitored continuously. Animal room and cage cleaning was performed according to KIT SOPs.

Test system

Vehicle:
not specified
Amount / concentration applied:
0.1 g
Duration of treatment / exposure:
Not specified (1 application)
Observation period (in vivo):
72 hours
Number of animals or in vitro replicates:
3
Details on study design:
To evaluate the eye irritation/corrosion of Zinc cyanide, 0.1g of the test item was applied in 3 healthy male New Zealand white rabbits. Mortality, clinical signs, body weight changes and eye response were measured during 72 hours after application.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: mean index of ocular irritation
Time point:
24/48/72 h
Score:
1.85
Max. score:
4.7
Reversibility:
fully reversible
Irritation parameter:
cornea opacity score
Time point:
24/48/72 h
Score:
0
Max. score:
0
Irritation parameter:
iris score
Time point:
24/48/72 h
Score:
0
Max. score:
0
Irritation parameter:
conjunctivae score
Time point:
24/48/72 h
Score:
0.3
Max. score:
1
Irritant / corrosive response data:
As a result of observation the application site until 72 hours after application of the test item, no abnormalities were observed in the cornea and iris of all animals. As a result of observation for redness, chemosis and discharge in the conjunctivia, slight redness, chemosis and discharge were observed due to the test item application.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Based on these results, the Index of Acute Ocular Irritation (IAOA) of the test item was calculated to be "4.7". According to the classification method for eye irritants, the test item was considered to be a minimal irritant.
Executive summary:

For that endpoint, one reliable study to assess eye irritation in vivo on the registered substance on rabbits was available. The study was performed according to the OECD Guideline 405 (Acute Eye Irritation / Corrosion).

Under the experimental conditions, the Index of Acute Ocular Irritation (IAOA) of Zinc cyanide batch n°10185145 was was calculated to be "4.7". Therefore, Zinc cyanide batch n°10185145 is not classified as eye irritant based on GHS criteria.

The validity criteria were successful and the study was therefore regarded as acceptable for that endpoint.