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EC number: 240-815-0
CAS number: 16752-77-5
An acute oral toxicity test (Up and Down Procedure) was conducted with
rats to determine the potential for the test substance to produce
toxicity from a single dose via the oral route in accordance with the
OECD Guideline 425 and U.S. EPA Guideline OPPTS 870.1100.
Based on an estimated LD50 of 32 mg/kg, a Main Test was conducted using
a default starting dose level of 10.1 mg/kg administered to one healthy
female rat by oral gavage. Following the Up and Down procedure, two
additional females were dosed at 10.1 mg/kg, one female was dosed at 3.2
mg/kg, three females were dosed at 32 mg/kg and one female was dosed at
101 mg/kg. Females were selected for the test because they are
frequently more sensitive to the toxicity of test compounds than males.
All animals were observed for mortality, signs of gross toxicity, and
behavioral changes at least once daily for 14 days after dosing or until
death occurred. Body weights were recorded prior to administration and
again on Days 7 and 14 (termination) following dosing or after death.
Necropsies were performed on all animals.
The animal dosed at 3.2 mg/kg survived and exhibited no clinical signs
or body weight loss during the study. No gross abnormalities were noted
for this animal when necropsied at the conclusion of the 14-day
One animal dosed at 10.1 mg/kg and two dosed at 32 mg/kg died within one
hour of test substance administration. Clinical signs noted in the
decedents prior to death included hypoactivity, tremors and/or prone
posture. The surviving rats from these dose groups exhibited clinical
signs including hypoactivity, tremors and/or piloerection on the day of
dosing. All surviving animals recovered from these signs within one day
post-dosing and appeared active and healthy for the remainder of the
14-day observation period, showing no body weight loss during the study.
Gross necropsy of the decedents revealed discoloration of the
intestines. No gross abnormalities were noted for any of the euthanized
animal necropsied at the conclusion of the 14-day observation period.
The rat dosed at 101 mg/kg died within 30 minutes of test substance
administration. Clinical signs noted in the decedent prior to death
included hypoactivity and prone posture. Gross necropsy of the decedent
revealed discoloration of the intestines.
Under the conditions of this study, the acute oral LD50 of the test
substance is estimated to be 17.98 mg/kg of body weight (based on
maximum likelihood) in female rats with a 95% profile-likelihood based
confidence interval of 0 mg/kg (lower) to greater than 20000 mg/kg
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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