Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Study from 1976 (no guidelines available at the time the study was performed).
Mixed groups of 5 male and female Sprague-Dawley strain albino rats were dosed with undiluted TS via gavage at 4 dose levels. Observations were made for toxic signs and the viscera of the test animals were examined macroscopically.

GLP compliance:

no

Remarks:

Study from 1976 (GLP was not compulsory at the time the study was performed).

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

EC Number:

905-459-9

Molecular formula:

C12H18

IUPAC Name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Test material form:

liquid

Details on test material:

Lot/batch No.: XF 958

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Initial body weights: 220 - 235 g (m) and 225 - 240 g (f)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

3160, 3980, 5010 or 6310 mg/kg bw

No. of animals per sex per dose:

mixed groups of 5 male and female rats

Control animals:

no

Details on study design:

Survivors were sacrificed 14 days after dosing.

Statistics:

No data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 900 mg/kg bw

95% CL:

>= 3 550 - <= 4 290

Mortality:

3160 mg/kg bw: 0/5
3980 mg/kg bw: 3/5
5010 mg/kg bw: 3/5
6310 mg/kg bw: 5/5

Survival time: 1 - 5 days, most deaths within 2 days.

Clinical signs:

other: reduced appetite and activity (1 - 3 days in survivors), increasing weakness, collapse and death.

Gross pathology:

Decedents: Haemorrhagic areas of the lungs, liver discoloration and acute GI tract inflammation.
Survivors: viscera appeared normal.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The oral LD50 value for male and female rats was 3900 mg/kg bw.

Executive summary:

In this older study mixed groups of 5 male and female Sprague-Dawley strain albino rats were dosed via gavage with undiluted TS at doses of 3160, 3980, 5010 or 6310 mg/kg bw. The oral LD50 value for male and female rats was given with 3900 mg/kg bw. Toxic signs included reduced appetite and activity, increasing weakness, collapse and death. In decedents, haemorrhagic areas of the lungs, liver discoloration and acute GI tract inflammation were noted, while in survivors viscera appeared normal.

As the other data indicated a LD50 higher than this one, it will be taken as the oral LD50 for mix DIPB