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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08/09/1988 - 14/02/1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study according to international guideline (OECD guideline 402) under GLP. Chemical identity of the test substance is not reported.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method B.3 (Acute Toxicity (Dermal))
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)
EC Number:
701-337-2
Cas Number:
not available
Molecular formula:
C30H24O8P2
IUPAC Name:
Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)
Details on test material:
- Name of test material (as cited in study report): CR 733-S
- Physical state: Liquid
- Storage condition of test material: At ambient temperature, in the dark, in a dry place

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River wige GmbH, Sulzfeld, FRG
- Age at study initiation: 9 weeks
- Fasting period before study: No
- Housing: polycarbonate cages containing purified sawdust (Woody Clean supplied by Broekman Institute, Someren, The Netherlands) as bedding material
- Diet (e.g. ad libitum): standard pelleted laboratory animal diet (RMH-B, pellet diameter 10 mm, Hope Farms, Woerden, The Netherlands), ad libitum
- Water (e.g. ad libitum): tap-water, ad libitum
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-20
- Humidity (%): 60-75
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: 08/09/1988 To: 22/09/1988

Administration / exposure

Type of coverage:
semiocclusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Type of wrap if used: surgical gauze, fixed with aluminium foil, flexible bandage and adhesive tape


REMOVAL OF TEST SUBSTANCE
- Washing (if done): Test substance gently removed with tissues, moistened with tap water
- Time after start of exposure: 24 hours


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.5 ml/kg bodyweight:
- Constant volume or concentration used: yes
Duration of exposure:
24 hours
Doses:
2000 mg/kg bodyweight
No. of animals per sex per dose:
5 males and 5 females
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing: weekly; clinical observations: daily (multiple times on day 0); observation of treated area on days 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, necropsy
Statistics:
No data

Results and discussion

Preliminary study:
Not relevant
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Mortality:
No mortality occured during the 14-day observation period.
Clinical signs:
Signs of toxicity included lethargy and blood nose encrustation on days 0 and 1, respectively.
The treated skin surface of animals showed no irritation.
Body weight:
All animals showed body weight gain during the study period. Males increased (on average) from 349 to 379 g during the 14-day period, female average weight increased from 234 to 244 g.
Gross pathology:
Macroscopic examination at necropsy of all animals at the end of the study did not reveal any changes that were considered to have arisen as a result of treatment.
Other findings:
No data

Any other information on results incl. tables

Table showing the mortality and clinical signs of rats in this study.

Day 0 Day
0:20 2:15 4:50 1 2 3 4 5 6 7 8 9 10 11 12 13 14
No abnormalities 10 10 0 9 10 10 10 10 10 10 10 10 10 10 10 10 10
Lethargy 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bloody nose encrustation 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Deaths 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute dermal toxicity (LD50) of CR 733-S towards rats is > 2000 mg/kg bodyweight.
Executive summary:

The acute dermal toxicity of CR 733 -S was determined according to OECD guideline 402 under GLP. Five male and 5 female rats were exposed to 2000 mg/kg bodyweight of the test substance for 24 hours and observed for 14 days. The LD50 was found to be > 2000 mg/kg bodyweight for both males and females.