Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was conducted under GLP conditions but not according to an OECD guideline. However, the design of the study was clear and proper, resembling the design of a standard acute toxicity study (limit test and intraperitoneal administration)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Acute toxicity in rats was studied after intraperitoneal injection of CR733S at a dose of 6500 mg/kg bw. Animals were observed for at least 4 hours and thereafter 14 days for signs of toxicity or mortality after dosing. At the end of 14 days, rat were subjected to gross necropsy examinations of visceral organs.

GLP compliance:

yes

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate

EC Number:

701-337-2

Cas Number:

not available

Molecular formula:

C30H24O8P2

IUPAC Name:

3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate

Details on test material:

- Name of test material (as cited in study report): CR733S
- Physical state: Liquid
- Analytical purity: No data
- Stability under test conditions: No data

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

unchanged (no vehicle)

Doses:

6500 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD0

Effect level:

6 500 mg/kg bw

Remarks on result:

other: No mortality observed

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 6 500 mg/kg bw

Remarks on result:

other: No mortality observed

Any other information on results incl. tables

No evidence of systemic toxicity:

- No remarkable clinical observations at any time during study

- No rats died during study

- All rats gained weight during the 14 day observation period

- Gross necropsy at end of 14 day observation period revealed increase in fat opacity throughout the mestery and related areas along the GI tract. No other remarkable consistent or distinct gross tissue or organ changes were noted.

Applicant's summary and conclusion

Conclusions:

Under the conditions of this study, an acute intraperitoneal dose CR733S of 6500 mg/kg bw did not show any signs of systemic toxicity in rats. Therefore, the LD50 is expected to be higher than 6500 mg/kg bw.

Executive summary:

Acute toxicity in rats was studied after intraperitoneal injection of CR733S at a dose of 6500 mg/kg bw. Animals were observed for at least 4 hours and thereafter 14 days for signs of toxicity or mortality after dosing. At the end of 14 days, rat were subjected to gross necropsy examinations of visceral organs.

Under the conditions of this study, an acute intraperitoneal dose CR733S of 6500 mg/kg bw did not show any signs of systemic toxicity in rats. Therefore, the LD50 is expected to be higher than 6500 mg/kg bw.