Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study was conducted under GLP conditions but not according to an OECD guideline. However, the design of the study was clear and proper, resembling the design of a standard acute toxicity study (limit test and intraperitoneal administration)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acute toxicity in rats was studied after intraperitoneal injection of CR733S at a dose of 6500 mg/kg bw. Animals were observed for at least 4 hours and thereafter 14 days for signs of toxicity or mortality after dosing. At the end of 14 days, rat were subjected to gross necropsy examinations of visceral organs.
GLP compliance:
yes
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)
EC Number:
701-337-2
Cas Number:
not available
Molecular formula:
C30H24O8P2
IUPAC Name:
Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)
Details on test material:
- Name of test material (as cited in study report): CR733S
- Physical state: Liquid
- Analytical purity: No data
- Stability under test conditions: No data

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
unchanged (no vehicle)
Doses:
6500 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD0
Effect level:
6 500 mg/kg bw
Remarks on result:
other: No mortality observed
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 6 500 mg/kg bw
Remarks on result:
other: No mortality observed

Any other information on results incl. tables

No evidence of systemic toxicity:

- No remarkable clinical observations at any time during study

- No rats died during study

- All rats gained weight during the 14 day observation period

- Gross necropsy at end of 14 day observation period revealed increase in fat opacity throughout the mestery and related areas along the GI tract. No other remarkable consistent or distinct gross tissue or organ changes were noted.

Applicant's summary and conclusion

Conclusions:
Under the conditions of this study, an acute intraperitoneal dose CR733S of 6500 mg/kg bw did not show any signs of systemic toxicity in rats. Therefore, the LD50 is expected to be higher than 6500 mg/kg bw.
Executive summary:

Acute toxicity in rats was studied after intraperitoneal injection of CR733S at a dose of 6500 mg/kg bw. Animals were observed for at least 4 hours and thereafter 14 days for signs of toxicity or mortality after dosing. At the end of 14 days, rat were subjected to gross necropsy examinations of visceral organs.

Under the conditions of this study, an acute intraperitoneal dose CR733S of 6500 mg/kg bw did not show any signs of systemic toxicity in rats. Therefore, the LD50 is expected to be higher than 6500 mg/kg bw.