1-Propene, 1,3,3,3-tetrafluoro-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling time: 0 and 48hr
- Sample storage conditions before analysis: Storaged at 2 - 10˚C and shipped at end of the study

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
In a sparging system, the test material was purged through test medium for different periods at a rate of approximately 20 mL per minute. The gas flow rate was assesseed using a soap- bubble flow meter. Air was simultaneoulsy purged to maintain acceptable levels of dissolved oxygen.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: In house laboratory culture with known history
- Age of the organisms: Young daphnia < 24 hours old from parental daphnids > 2 weeks of age

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

pH:

Start of the test: pH 7.9 - 8.0
End of the test (48h): pH 7.8 - 8.0

Dissolved oxygen:

Start of test: 6.0 - 9.6
End of the test (48 h): 6.2 - 8.8

Nominal and measured concentrations:

Nominal concentration - number of minutes of purging test material at 20 mL/min (4, 7.5, 15, 30 and 60 minutes)
Measured - below limit except at 15 min (50 mg/L); 30 min (164 mg/L), 60 min (347 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: Closed
- Material, size, headspace, fill volume: 100 mL, all glass, filled up to rim
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 160 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: None
- Other biological observations: N one
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Immobilization:
5% at 4 mg/L
10% at 164 mg/L
45% at 347 mg/L

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: Yes
- EC50/LC50: 48 hr - 0.51 mg/L

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test with Daphnia magna, performed in accordance with OECD TG 202 and EU method C2, the 48hr EC50 was determined to be > 160 mg/L, based on mean measured concentrations.

Description of key information

In an acute toxicity test with Daphnia magna, performed in accordance with OECD TG 202 and EU method C2, the 48hr EC50 was determined to be > 160 mg/L, based on mean measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 160 mg/L