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EC number: 471-480-0 | CAS number: 1645-83-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- -
- EC Number:
- 471-480-0
- EC Name:
- -
- Cas Number:
- 1645-83-6
- Molecular formula:
- Hill formula: C3H2F4 CAS formula: C3H2F4
- IUPAC Name:
- (1E)-1,3,3,3-tetrafluoroprop-1-ene
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- lymphocytes: human
- Cytokinesis block (if used):
- Colcemid
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 0, 10, 20, 40, 60, and 76 % in the atmosphere
Controls
- Untreated negative controls:
- yes
- Remarks:
- sham exposed
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- mitomycin C
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: substance is a liquified gas. Lymphocytes were exposed in a modular incubator chamber in atmospheric concnetrations of 19% O2, 5% CO2, and up to 76% test substance
DURATION
- Preincubation period: 48hrs
- Exposure duration: 4hrs
- Expression time (cells in growth medium):20 or 44 hrs
- Fixation time (start of exposure up to fixation or harvest of cells): 26-50 hrs
SPINDLE INHIBITOR (cytogenetic assays): colcemid
STAIN (for cytogenetic assays): Geimsa
NUMBER OF REPLICATIONS:2
NUMBER OF CELLS EVALUATED:100
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index
- Evaluation criteria:
- Biological relevance and statistical significance are both considered in the evaluation of the results.
- Statistics:
- Fischer's exact probability test (two sided)
Results and discussion
Test results
- Species / strain:
- lymphocytes: human
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- slight cytotoxicity at highest concentration with metabolic activation
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Test substance did not induce a statistically significant increase in the number of aberrant cells at any concentration or time point analysed with compared to the number of aberrant cells observed in the negative (control air) control cultures.
The positive controls mitomycin C (without S9) and cyclophosphamide (with S9) induced the expected statistically significant increase in the incidence of structural chromosomes.
Applicant's summary and conclusion
- Conclusions:
Under the conditions of this study, the test substance was not clastogenic in cultured human lymphocytes.
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