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EC number: 946-248-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From December 21, 1982 to January 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Citronellyl 3-methylcrotonate
- EC Number:
- 244-019-4
- EC Name:
- Citronellyl 3-methylcrotonate
- Cas Number:
- 20770-40-5
- Molecular formula:
- C15H26O2
- IUPAC Name:
- 3,7-dimethyloct-6-en-1-yl 3-methylbut-2-enoate
- Reference substance name:
- 3,7-dimethyloctyl 3-methyl-2-butenoate
- EC Number:
- 275-360-7
- EC Name:
- 3,7-dimethyloctyl 3-methyl-2-butenoate
- Cas Number:
- 71383-07-8
- Molecular formula:
- C15H28O2
- IUPAC Name:
- 3,7-dimethyloctyl 3-methylbut-2-enoate
- Reference substance name:
- 3,7-dimethyloct-7-en-1-yl 3-methylbut-2-enoate
- Cas Number:
- 74499-48-2
- Molecular formula:
- C15H26O2
- IUPAC Name:
- 3,7-dimethyloct-7-en-1-yl 3-methylbut-2-enoate
- Test material form:
- liquid
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- A sample of the test product, a colourless liquid with a mild flavour, designated Sinodor (X-09648, 13.12.82), was received from the sponsor on December 21, 1982. The test material was dissolved in ethanol just prior to use.
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
S9 mix is prepared by mixing the thawed S9 with a NADPH generating system. The final concentrations of the various ingredients in the S9 mix are:
MgCl2 8 mM
KCl 33 mM
G6P 5 mM
NADP 4 mM
Sodium phosphate pH 7.4 100 mM
S9 10%
The S9 mix is prepared just prior to use with cold sterile solutions and is kept in ice water before and during use - Test concentrations with justification for top dose:
- A preliminary test was carried out to assess the chemical toxicity of the test substance for the bacteria. The results show that 10 mg of the test substance per plate was slightly toxic whereas 1 mg per plate did not show a growth-inhibiting effect. However, at a dose of 1 mg per plate, the compound precipitated in the plate. Therefore 500 micrograms per plate was chosen as the highest dose level for the mutagenicity study.
Appropriate test solutions in ethanol containing 0, 0.06, 0.19, 0.56, 1.67 and 5.00 mg/ml were prepared immediately before use. - Vehicle / solvent:
- Ethanol
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- other: hycanthone methanesulphonate (12.5 micrograms per 0.1 ml water per plate) for strains TA 1537, TA 1538 and TA 98 without S9 mix
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (0.5 micrograms per 0.1 ml DMSO per plate) for TA 1535, TA 1538, TA 98, TA 100 in the presence of S9 mix. For TA 1537 2.0 micrograms per plate was used in the presence of S9 mix.
- Details on test system and experimental conditions:
- Preparation and storage of stock cultures:
A fresh culture of each strain is prepared by inoculating nutrient broth with bacteria of the strain in question and incubating the broth for 16h (overnight) at 37°C while shaking. The cultures are then mixed with DMSO to a final DMSO concentration of 8.75%. Subsequently 0.5 ml portions are pipetted into sterile polypropylene vials which are then quickly frozen on dry ice and stored at -80°C.
Part of each culture is retained and examined for the number of spontaneous revertants, histidine requirement and sensitivity to ampicilline, crystal violet and UV radiation. In addition the reversion induced by reference mutagens is determined.
Preparation of cultures for the tests:
To obtain a culture for the mutagenicity test, nutrient broth is inoculated with a thawed aliquot of the stock culture in question (0.1 ml per 10 ml nutrient bouillon). The broth is incubated for 16h (overnight) at 37°C while shaking. The viable count of each culture is determined by plating appropriate dilutions of the culture on nutrient broth agar plates. The cultures are subsequently stored in a refrigerator at 5°C until use, but no longer than 5 days. - Evaluation criteria:
- A positive response in the assay system is taken to be a two-fold or greater increase in the mean number of revertant colonies appearing in the test plates over and above the background spontaneous reversion rate observed with the solvent, together with evidence of a dose response.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: High concentration based on precipitation.
Applicant's summary and conclusion
- Conclusions:
- It is concluded that SINODOR does not show any mutagenic activity in the Salmonella/mammalian microsome mutagenicity test under the conditions employed in this evaluation.
- Executive summary:
Sinodor was examined for mutagenic activity in the Ames test using the histidine requiring S.typhimurium mutants TA 1535, TA 1537, TA 1538, TA98 and TA100 as indicator strains and a liver microsome fraction of Aroclor-induced rats for metabolic activation.
It is concluded that SINODOR does not show any mutagenic activity in the Salmonella/mammalian microsome mutagenicity test under the conditions employed in this evaluation.
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