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EC number: 222-468-7 | CAS number: 3483-12-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Particle size distribution (Granulometry)
Administrative data
- Endpoint:
- particle size distribution (granulometry)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 January - 3 February 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 110 (Particle Size Distribution / Fibre Length and Diameter Distributions - Method A: Particle Size Distribution (effective hydrodynamic radius)
- Version / remarks:
- 1981
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 13320 (Particle size analysis - Laser diffraction methods)
- Version / remarks:
- 2009
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 830.7520 (Particle Size, Fiber Length, and Diameter Distribution)
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- Laser scattering/diffraction
- Type of distribution:
- volumetric distribution
Test material
- Reference substance name:
- (R*,R*)-1,4-dimercaptobutane-2,3-diol
- EC Number:
- 222-468-7
- EC Name:
- (R*,R*)-1,4-dimercaptobutane-2,3-diol
- Cas Number:
- 3483-12-3
- Molecular formula:
- C4H10O2S2
- IUPAC Name:
- 1,4-disulfanylbutane-2,3-diol
Constituent 1
- Specific details on test material used for the study:
- Batch No.: 44606300 / 50774
Storage Conditions: 2°C to 8°C, under nitrogen
Results and discussion
Particle sizeopen allclose all
- Key result
- Percentile:
- D10
- Mean:
- 20.5 µm
- St. dev.:
- 0.48
- Remarks on result:
- other: Average result calculated from the results of two test series
- Key result
- Percentile:
- D50
- Mean:
- 556.9 µm
- St. dev.:
- 0.78
- Remarks on result:
- other: Average result calculated from the results of two test series
- Key result
- Percentile:
- D90
- Mean:
- 1 147.1 µm
- St. dev.:
- 0.41
- Remarks on result:
- other: Average result calculated from the results of two test series
Any other information on results incl. tables
Light Microscopy
At 5x and 20x magnifications bigger particles can be observed. Examining the 50x magnification, the test item consists of crystalline particles.
Particle Size Distribution
Two test series of 3 measurements each were performed with the test item.
Test series 1:
Percentile |
Particle size (µm) |
Standard deviation |
D10 |
21.0 |
0.96 |
D50 |
556.1 |
3.79 |
D90 |
1147.5 |
3.64 |
Test series 2:
Percentile |
Particle size (µm) |
Standard deviation |
D10 |
20.0 |
1.13 |
D50 |
557.6 |
7.88 |
D90 |
1146.7 |
7.83 |
Applicant's summary and conclusion
- Conclusions:
- The test item 1,4 Dithiothreitol (CAS no. 3483-12-3) has a coarse fraction > 2000 µm of 29.2 wt.-%. The fine fraction has a median particle size of D50 = 556.9 µm, which was determined by laser diffraction measurement according to OECD Test Guideline 110 (1981), ISO 13320 (2009) and EPA OCSPP Guideline 830.7520 (1996).
- Executive summary:
The test item1,4-Dithiothreitol (CAS no.3483-12-3) has a coarse fraction > 2000 µm of 29.2 wt.-%. The fine fraction has a median particle size of D50 = 556.9 µm, which was determined by laser diffraction measurement according to OECD Test Guideline 110 (1981), ISO 13320 (2009) and EPA OCSPP Guideline 830.7520 (1996).
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