(R*,R*)-1,4-dimercaptobutane-2,3-diol

Particle size distribution (Granulometry)

Administrative data

Endpoint:

particle size distribution (granulometry)

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 January - 3 February 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 110 (Particle Size Distribution / Fibre Length and Diameter Distributions - Method A: Particle Size Distribution (effective hydrodynamic radius)

Version / remarks:

1981

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

ISO 13320 (Particle size analysis - Laser diffraction methods)

Version / remarks:

2009

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7520 (Particle Size, Fiber Length, and Diameter Distribution)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

Laser scattering/diffraction

Type of distribution:

volumetric distribution

Test material

Test material information

Constituent 1

Reference substance name:

(R*,R*)-1,4-dimercaptobutane-2,3-diol

EC Number:

222-468-7

EC Name:

(R*,R*)-1,4-dimercaptobutane-2,3-diol

Cas Number:

3483-12-3

Molecular formula:

C4H10O2S2

IUPAC Name:

1,4-disulfanylbutane-2,3-diol

Specific details on test material used for the study:

Batch No.: 44606300 / 50774
Storage Conditions: 2°C to 8°C, under nitrogen

Results and discussion

Particle size

Particle size 1

Key result

Percentile:

D10

Mean:

20.5 µm

St. dev.:

0.48

Remarks on result:

other: Average result calculated from the results of two test series

Particle size 2

Key result

Percentile:

D50

Mean:

556.9 µm

St. dev.:

0.78

Remarks on result:

other: Average result calculated from the results of two test series

Particle size 3

Key result

Percentile:

D90

Mean:

1 147.1 µm

St. dev.:

0.41

Remarks on result:

other: Average result calculated from the results of two test series

Any other information on results incl. tables

Light Microscopy

At 5x and 20x magnifications bigger particles can be observed. Examining the 50x magnification, the test item consists of crystalline particles.

 Particle Size Distribution

Two test series of 3 measurements each were performed with the test item.

Test series 1:

Percentile	Particle size (µm)	Standard deviation
D10	21.0	0.96
D50	556.1	3.79
D90	1147.5	3.64

Test series 2:

Percentile	Particle size (µm)	Standard deviation
D10	20.0	1.13
D50	557.6	7.88
D90	1146.7	7.83

Applicant's summary and conclusion

Conclusions:

The test item 1,4 Dithiothreitol (CAS no. 3483-12-3) has a coarse fraction > 2000 µm of 29.2 wt.-%. The fine fraction has a median particle size of D50 = 556.9 µm, which was determined by laser diffraction measurement according to OECD Test Guideline 110 (1981), ISO 13320 (2009) and EPA OCSPP Guideline 830.7520 (1996).

Executive summary:

The test item1,4-Dithiothreitol (CAS no.3483-12-3) has a coarse fraction > 2000 µm of 29.2 wt.-%. The fine fraction has a median particle size of D50 = 556.9 µm, which was determined by laser diffraction measurement according to OECD Test Guideline 110 (1981), ISO 13320 (2009) and EPA OCSPP Guideline 830.7520 (1996).