Registration Dossier
Registration Dossier
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EC number: 950-250-8 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of butyl phenyl hydrogen phosphate and diphenyl hydrogen phosphate and phenyl dihydrogen phosphate
- EC Number:
- 950-250-8
- IUPAC Name:
- Reaction mass of butyl phenyl hydrogen phosphate and diphenyl hydrogen phosphate and phenyl dihydrogen phosphate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal waste water treatment plant Mannheim, Germany
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Initial conc.:
- 38 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2 °C
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no
SAMPLING
- Sampling frequency: every other day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control
Reference substance
- Reference substance:
- aniline
- Remarks:
- 20 mg/L TOC
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 80
- Sampling time:
- 28 d
- Details on results:
- Phenyl acid phosphate was readily biodegradable under the
conditions of this test over the 28-day exposure to activated
sludge according to OECD guideline 301B.
The required pass level for ready biodegradability i.e., at least
60% of the theoretical CO2 production in a 10-day window
exceeding 10% degradation within the 28-day period of the test
was reached.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
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