dialkyl-methyldihydro-heteropolycycle

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020-03-31 to 2020-06-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

Based on the predicted moisture content of artificial soil approximately 30 % and the purity of the test substance was 98.5%, respectively 586 mg of test substance was weighed and mixed well with quartz sand to a total weight of about 10 g by grinding. Each mixture was then mixed with about 740 g wet artificial soil to obtain test substance concentrations of 1000 mg/kg (dry weight of atrtificial soil). Four replicates were prepared. Blank control was prepared by mixing about 10 g of quartz sand and about 740 g wet artificial soil, with four replicates.
The above test media (750 g wet weight) was transferred into 1L glass beakers. Ten healthy earthwonns which had been washed and dried with filter paper before exposure were placed randomly on the surface of artificial soil in each container. These earthworms were placed on the surface and allowed to burrow into the test medium by themselves. The containers were covered with perforated plastic film to prevent the artificial soil from drying.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

The earthworms which were used in the test were purchased on February 20, 2020, and all earthwonns have been raised in the laboratory for 40 days before use.
Before the test, earthworms were acclimated to the study conditions for about 24 hours in the aitificial soil.

Batch No. EEf20200220-I
Weight 521 mg - 596 mg per earthwom
Age Older than 2 months

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20°C ± 2°C

pH:

5.84 (at the beginning of the test)
6.32 - 6.37 (at the end of the test)

Moisture:

29.7 % (dry weight, at the beginning of the test)
28.2% - 28.8% (dry weight, at the end of the test)

Details on test conditions:

The test systems were put into an incubator to cultivate. During the test, the light intensity was 400 1x - 800 1x with continuous light, the environment temperature was 20°C±2°C, and the humidity was 65% - 100%. The test duration was 14 days.

Nominal and measured concentrations:

Nominal: 1000 mg/kg (dry weight of atiificial soil).

Reference substance (positive control):

yes

Remarks:

chloroacetamide (C2H4ClNO)

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

After 7 days and 14 days exposure, the mortalities of earthworms in the blank control were both 0%, and in the 1000 mg/kg (dry weight of artificial soil) test group were both 0%.

Results with reference substance (positive control):

The sensitivity test was carried out with the reference substance chloroacetamide (C2H4ClNO) based on the artificial soil test of the same batch Eisenia foetida. The 14 d-LC50 of chloroacetamide was 27.6 mg/kg (dry
weight of artificial soil), 95% confidence limit was 25.7 mg/kg - 29.7 mg/kg.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The mortality of earthworms in blank control was 0% at the end of the test, which fulfilled the criteria for the validity of not exceeding 10%.

Conclusions:

Under the test conditions, the result for the 14 d median lethal concentration (LC50) was greater than 1000 mg/kg (dry weight of artificial soil).

Executive summary:

The artificial soil test involves keeping earthworms in samples of a precisely defined artificial soil, in which 1000 mg/kg (dry weight of artificial soil) of the test substance had been applied.

Mortality was assessed 7 days and I 4 days after application. Then the acute toxicity of the test substance was detennined by the results of the mortality.

After 7 days and 14 days exposure, the mortalities of earthworms in the blank control were both 0%, and in the 1000 mg/kg (dry weight of artificial soil) test group were both 0%.

Under the test conditions, the result for the 14 d median lethal concentration (LC50) was greater than 1000 mg/kg ( dry weight of artificial soil ).