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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Description of key information

ErC50 (72h) > 100 mg/l

Key value for chemical safety assessment

Additional information

The influence of the test item 3,4-dichlorobenzotrichloride on the growth of the green algal species Pseudokirchneriella subcapitata, formerly known as Selenastrum capricornutum, was investigated in a 72-hour limit test according to the OECD Guideline No. 201, 2006 and to Council Regulation EC 440/2008 (C.3).

On this purpose, exponentially growing test algae were exposed for 72 hours to an aqueous test medium solution at the only nominal concentration of 100.0 mg/L, under defined conditions. Besides this concentration, a negative control without the test item was also prepared to check the acceptability of the algal inoculum.Algal cell density was measured every 24 hours by fluorescent reading with a spectrofluorophotometer in few millilitre samples taken from each test concentration replicate and from controls.

The pH of the test medium after preparation was 7.23 for test item and 7.96 for control.

The room temperature was in the range 24.6 – 25.3 °C during the test period, according to the OECD’s recommended range (24.0± 2 °C).Light intensity during the test period was in the range 4078 - 4156 Lux (within the range 4000 - 6000 Lux).


In the negative control the cell density increased on average by a factor of 191. This value complies with the validity criteria of the test according to the mentioned guidelines, such as the coefficient of variation of daily growth rates (32.9%) and the coefficient of variation of average growth in replicates control cultures during the test period (3.7%).

In the test item solution, at the nominal concentration of 100.0 mg/L, no significant biological effects were shown in comparison with negative control for both the endpoints, yield and growth rate, after 72 hours of exposure (Bonferroni adjusted test).

Therefore, the 72-h EyC10,EyC20, EyC50, and 72-h ErC10,ErC20,ErC50, were determined to be higher than the nominal tested concentration (100 mg/L).