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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
In the absence of data on the microbial toxicity the test concentration used in the ready biodegradation study can be used as the NOEC to fill the ‘Toxicity to microbes’ endpoint requirements (ECHA, 2017, R7b). Please refer to section 5.2.1 for the full study summary.
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: EU Method C.6 (Degradation: Chemical Oxygen Demand)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
XRD 535 Me
Lot # : GHD-2413-21
Purity: 93.9%
Test organisms (species):
activated sludge
Details on inoculum:
- Source of inoculum/activated sludge: The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage
- Preparation of inoculum for exposure: The sample was allowed to settle and the supernatant filtered through Whatman GFA filter paper (first 250 mL discarded)
Limit test:
no
Total exposure duration:
28 d
Test temperature:
20 ± 1°C
Nominal and measured concentrations:
3 mg/L
Details on test conditions:
TEST CONDITIONS
- Composition of medium: Standard nutrient medium (solution 1: KH2PO4, K2HPO4, Na2H PO4.2H2O, NH4Cl; solution 2: MgSO4.7H2O; solution 3: CaCl2; Solution 4: FeCl3.6H2O)
- Test temperature: 20 ± 1°C
- Continuous darkness: Yes

TEST SYSTEM
- Culturing apparatus: 280 mL BOD bottles (darkened glass) with ground glass stoppers
- Number of culture flasks/concentration: 2
- Test performed in closed vessels due to significant volatility of test substance: Closed vessels

SAMPLING
- Sampling frequency: 0, 5, 15 or 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: Inoculated dilution water; inoculated dilution water and filter paper
- Abiotic sterile control: Non-inoculated dilution water
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: biodegradability
Conclusions:
The test substance attained between 8-11% degradation after 28 days and therefore cannot be termed as readily biodegradable.
Executive summary:

The ready biodegradability of the test substance was assessed using a closed bottle procedure according to EEC directive C6 and OECD guideline 301D.

The test substance attained between 8-11% degradation after 28 days and therefore cannot be termed as readily biodegradable.

The standard substances, sodium benzoate and aniline, attained 81-83% and 59% degradation respectively within 28 days.

Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits.

Description of key information

28-day NOEC (activated sludge) = 3 mg/L; OECD 301 D, EU Method C.6; Reliability = 1

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
3 mg/L

Additional information