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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Feb 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed according to guidelines and under GLP. No analytical monitoring of substance.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Direct dispersion in de-chlorinated tap water. The substance was known to adsorb to surfaces, so glass vessels were first saturated with substance, by soaking overnight with solution.
Test organisms (species):
Daphnia magna
Details on test organisms:
Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee, {I.R.CH.A}, France.
Culture: At 21°C in polypropylene vessels containing two litres of dechlorinated and aged tap water. Cultures were fed daily with a suspension of mixed algae {predominantly Chlorella spp.}. Culture conditions ensure that reproduction is by parthenogenesis.
Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
50 mg/L as CaCO3
Test temperature:
22 °C
pH:
7.9 - 9.7
Dissolved oxygen:
7.9 - 8.6 mg/L
Nominal and measured concentrations:
3.2, 5.6, 10, 18, 32, 56, 100, 180 and 320 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass jars each containing 200 ml test solution and covered to reduce evaporation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
-
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: De-chlorinated tap-water

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16h light 8 h dark
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
64 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL 56 - 75 mg/L
Conclusions:
The substance can be considered harmful to Daphnia Magna.
Executive summary:

In a Acute toxicity test according to OECD 202 with Daphnia Magna, a 48 h EC50 of 64 mg/L was observed.

Description of key information

The substance can be considered harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
64 mg/L

Additional information

In a 48 h acute toxicity study with the substance a EC50 value of 64 mg/L was obtained. For diethanolamine which is a large component of the multiconstituent substance a similar value is available.