1-(3,5-Dichloro-4-fluoro-phenyl)-2,2,2-trifluoro-ethanone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From April 20 to April 22, 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Lot number: 1930461
Purity: 97.3%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 2.0, 3.6, 6.3, 11.3 and 20 mg/L
- Sampling method: the concentrations of the test chemical were analyzed in 0 hour fresh solution and 48 hours old solutions.
- Sample storage conditions before analysis: stored in refrigerator (2-8 °C)

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0501 g test item was weighed and dissolved in 500 mL of test water by magnetic stirring for about 4 hours. After the test item was dissolved completely, the solution of 100 mg/L was used to dilute the test solutions. Pipette 10.0, 18.0, 31.5, 56.5 and 100 mL of the solution of 100 mg/L and make up to 500 mL with test water. The solutions were mixed thoroughly, resulting in test solutions at 2.0, 3.6, 6.3, 11.3 and 20 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone:
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD): less than 24 hours old and not the first brood progeny.
- Source: Daphnia of stock culture was originally obtained from Applied Chemistry Department Pesticide Environmental Evaluation Laboratory of China Agricultural University.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

140 to 250 mg/L (as CaCO3)

Test temperature:

18~22oC

pH:

6.0 to 8.5

Dissolved oxygen:

not less than 3 mg/L during the test period

Nominal and measured concentrations:

Nominal concentrations: 2.0, 3.6, 6.3, 11.3 and 20 mg/L;
Measured concentrations: 1.55, 2.43, 4.37, 8.13 and 14.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass conical flask and the flasks were stoppered
- Volume of solution: About 120 mL per flask
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was tap water after being treated with Aquapro Pure Water System.
- Total organic carbon: < 2 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hr light: 8 hr dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Biological observation: Observation intervals: 24 and 48 hours after exposure
Water quality and physicochemical measurements: at the beginning and end of the test

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 1, 2, 10, 20, 50 and 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 48 hours, no immobilization or any toxic signs were observed in the control group and the test groups of 1 and 2 mg/L. 100% immobilization occurred in the test group of 20, 50 and 100 mg/L after 48 hours.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.59 mg/L

95% CI:

2.93 - 4.41

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

During the 48-hour exposure period, no immobilization and abnormal behavior or appearance was observed in the control group and the test group of 2.0 mg/L, the daphnids exposed to 3.6, 6.3, 11.3 and 20 mg/L test groups showed abnormal behavior such as immobilization, 100% inhibition occurred in 20 mg/L test group after 48 hours.

Results with reference substance (positive control):

EC50-24 h of potassium dichromate to Daphnia magna was 1.44 mg/L, and the 95% confidence limits was 1.23 ~ 1.69 mg/L. The results met the recommended criterion in OECD Guideline No. 202: EC50-24 h of potassium dichromate is within the range of 0.6 mg/L to 2.1 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of the test item PF-06458663 (Trifluoroketone) to Daphnia magna was calculated to be 3.59 mg/L.

Executive summary:

A Daphnia magna acute immobilization test of PF-06458663 (Trifluoroketone) was conducted with Daphnia magna over a 48-hour exposure period, according to OECD Guideline 202 under GLP.

According to the results of test and based on the geometric mean of determined concentrations, the 48-hour EC₅₀ of the test item PF-06458663 (Trifluoroketone) to Daphnia magna was calculated to be 3.59 mg/L.

Description of key information

The 48-hour EC₅₀ of the test item PF-06458663 (Trifluoroketone) to Daphnia magna was calculated to be 3.59 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

3.59 mg/L

Additional information

A Daphnia magna acute immobilization test of PF-06458663 (Trifluoroketone) was conducted with Daphnia magna over a 48-hour exposure period, according to OECD Guideline 202 under GLP.

According to the results of test and based on the geometric mean of determined concentrations, the 48-hour EC₅₀ of the test item PF-06458663 (Trifluoroketone) to Daphnia magna was calculated to be 3.59 mg/L.