Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study according to GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium sarcosinate
EC Number:
224-338-5
EC Name:
Sodium sarcosinate
Cas Number:
4316-73-8
Molecular formula:
C3H6NNaO2
IUPAC Name:
sodium (methylamino)acetate
Details on test material:
- Test substance: sodium sarcosinate
- CAS No.: 4316-73-8

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source: Activated sludge from laboratory wastewater plants treating municipal sewage .
- Concentration of dry substance: 1 g/L .

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2°C
Details on test conditions:
TEST CONDITIONS
- test temperature: 20 °C
- test vessels: Erlenmeyer flask
- test volume: 250 ml
- synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- oxygen concentration during aeration: >2.5 mg/l
- oxygen concentration immediately before measurement: >6.5 mg/l
- duration of the measurement of oxygen consumption: 6-10 min

VALIDITY CRITERIA
- deviation of blank controls <15 %: yes
- EC50 of 3,5-dichlorophenol in the range of 5-30 mg/l: yes
- test is valid: yes
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables


No significant inhibition of respiration was measured up to
the highest tested concentration of 1000 mg/l (nominal).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes