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EC number: 224-338-5 | CAS number: 4316-73-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- Sodium sarcosinate
- EC Number:
- 224-338-5
- EC Name:
- Sodium sarcosinate
- Cas Number:
- 4316-73-8
- Molecular formula:
- C3H6NNaO2
- IUPAC Name:
- sodium (methylamino)acetate
- Details on test material:
- - Test substance: sodium sarcosinate
- CAS No.: 4316-73-8
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: Activated sludge from laboratory wastewater plants treating municipal sewage .
- Concentration of dry substance: 1 g/L .
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +/- 2°C
- Details on test conditions:
- TEST CONDITIONS
- test temperature: 20 °C
- test vessels: Erlenmeyer flask
- test volume: 250 ml
- synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- oxygen concentration during aeration: >2.5 mg/l
- oxygen concentration immediately before measurement: >6.5 mg/l
- duration of the measurement of oxygen consumption: 6-10 min
VALIDITY CRITERIA
- deviation of blank controls <15 %: yes
- EC50 of 3,5-dichlorophenol in the range of 5-30 mg/l: yes
- test is valid: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Any other information on results incl. tables
No significant inhibition of respiration was measured up to
the highest tested concentration of 1000 mg/l (nominal).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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