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Diss Factsheets
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EC number: 224-338-5 | CAS number: 4316-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD test guideline 431
- Principles of method if other than guideline:
- The present test is based on the experience that corrosive chemicals produce cytotoxicity in human reconstructed epidermis after a short term topical exposure. The test is designed to predict skin corrosion potential of a chemical by using the three dimensional human epidermis model EpiDermTM. After application of the test material to the stratum corneum surface of the EpiDermTM tissue for 3 minutes and 1 hour the induced cytotoxicity (=loss of viability) is measured by a colorimetric assay. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity. The mitochondrial dehydrogenase reduces the yellow colored water-soluble 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to the insoluble blue colored formazan. After isopropanol extraction of the formazan from the tissue the optical density of the extract is determined spectrophotometrically. Optical density of the extracts of test substance treated tissues is compared to negative control values from tissue treated with doubly distilled water and expressed as relative tissue viability.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Sodium sarcosinate
- EC Number:
- 224-338-5
- EC Name:
- Sodium sarcosinate
- Cas Number:
- 4316-73-8
- Molecular formula:
- C3H6NNaO2
- IUPAC Name:
- sodium (methylamino)acetate
Constituent 1
Test animals
- Species:
- other: EpiDerm Corrosivity-Test in vitro
Test system
- Amount / concentration applied:
- undiluted
- Duration of treatment / exposure:
- 3 min, 1 h
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 3 min
- Value:
- ca. 71
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 1 hour
- Value:
- ca. 16
Any other information on results incl. tables
Exposure: 3 min | ||||||
Test-Article | OD570 tissue 1 | OD570 Tissue 2 | OD570 Killed tissue control | mean OD570 | mean OD570 killed tissue control corrected | Viability [% of negative control] |
Negative Control | 1.877 | 2.001 | 0.219 | 1.939 | - | 100 |
Sarcosine-Na solution | 1.741 | 0.996 | 0.233 | 1.369 | - | 71 |
Positive Control | 0.463 | 0.397 | 0.430 | - | 22 | |
Exposure: 1 h | ||||||
Test-Article | OD570 tissue 1 | OD570 Tissue 2 | OD570 Killed tissue control | mean OD570 | mean OD570 killed tissue control corrected | Viability [% of negative control] |
Negative Control | 1.879 | 1.895 | 0.221 | 1.887 | - | 100 |
Sarkosine-Na solution | 0.285 | 0.306 | 0.266 | 0.296 | - | 16 |
Positive Control | 0.143 | 0.341 | - | 0.242 | - | 13 |
Applicant's summary and conclusion
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