Reaction mass of Disodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](hydroxy)chromate(2-) and Trisodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](dihydroxy)chromate(3-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 December 1994 to 09 November 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT-20044/B
Batch-Nr.: 99
Appearance: solid
Solubility: 60g/l [in water]
Storage: room temperature
Expiration date: 07/99

Analytical monitoring:

yes

Details on sampling:

Test medium samples for the analytical determinations were taken from the approximate center of the test vessels ( after mixing the medium ) and kept at -18 °C to -25 °C until analysis. The samples of the test substance at the beginning and at the end of the test were sent to the PE Analytical Services K, Textile Dyes Division.

Vehicle:

yes

Remarks:

Tween 80 (Polyoxyethylensorbitanmonooleat)

Test organisms (species):

Daphnia magna

Details on test organisms:

The organism used in this test system is the species Daphnia magna. The daphnids were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.
Breeding:
- Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22 °C. The water was renewed partially three times per week. The daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food was consumed within 24h.
Pretreatment:
- 24 hours before the start of the exposure reproductive daphnids were separated from the young daphnids by sieving all individuals through a 1000 µm sieve.
For each test concentration, 20 daphnids divided in two groups of 10 animals were used.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

178 mg/l CaCO3

Test temperature:

21±1 °C

pH:

7.9 to 8

Dissolved oxygen:

95 to 98

Nominal and measured concentrations:

Nominal: 45, 100, 220 mg/l
Measured:
Actual mean conc, mg/l: 47, 105 and 225

Reference substance (positive control):

yes

Remarks:

Potassium dichmmate.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 225 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

225 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations.

Results with reference substance (positive control):

The EC50 value should be in the range of 0.8-1.5mg/l. Last reference control at October 12, 1994 ; EC50 1.01 mg/l.

Determination of the EC - values

A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The NOEC and EC50 value after 48 hours were determined to be 225 mg/l.

The EC50 and the EC-100 value could not be determined, but are clearly higher than 225 mg/l.

 

Number of immobilized daphnids after 24 and 48 hours

Actual conc. of test substance mg/l	No. of animals	No. of responses		% immobility
		24 h	48 h	24 h	48 h
Blank	10	0	0	0	0
	10	0	0
Vehicle	10	0	0	0	0
	10	0	0
47	10	0	0	0	0
	10	0	0
105	10	0	0	0	0
	10	0	0
225	10	0	0	0	0
	10	0	0

 

Validity criteria fulfilled:

yes

Conclusions:

The NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.

Executive summary:

The short term toxicity to daphnids was carried out with FAT 20044/B according to OECD guideline 202. A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test, the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The EC50 and the EC100 value could not be determined, but are clearly higher than 225 mg/l. Based on the study results, The NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.

Description of key information

The short-term toxicity to daphnids was carried out with FAT 20044/B according to OECD guideline 202. A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test, the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The EC50 and the EC100 value could not be determined, but are clearly higher than 225 mg/l. Based on the study results, the NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

225 mg/L