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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: test performed prior to guideline availability but with procedures similar to standard methods and with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
other: Published toxicological report
Title:
Toxicological Profile for Hexachloroethane
Author:
Agency for Toxic Substances and Disease Registry (ATSDR)
Year:
1997
Bibliographic source:
Not reported
Reference Type:
publication
Title:
The toxicity of hexachloroethane in laboratory animals.
Author:
Weeks MH, Angerhofer RA, Bishop R, Thomasino J, Pope CR.
Year:
1979
Bibliographic source:
Am Ind Hyg Assoc J. 1979 Mar;40(3):187-99. doi: 10.1080/15298667991429499. PMID: 495459.
Reference Type:
study report
Title:
Unnamed
Year:
1978

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: determine the acute oral toxicity of the test material by dosing rats with a single administration and monitoring mortality over a 14-day observation period.
- Short description of test conditions: the test compound was dissolved in corn oil (50% w/v) or methylcellulose (5% w/v). Solutions were administered by stomach tube to male and female rats. The oral LD50 were calculated after a 14-day observation period.
- Parameters analysed / observed: mortality
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexachloroethane
EC Number:
200-666-4
EC Name:
Hexachloroethane
Cas Number:
67-72-1
Molecular formula:
C2Cl6
IUPAC Name:
hexachloroethane
Test material form:
solid: crystalline
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: Hummel Chemical Company, specifications MIL-H-235B, national stock number 6810-00-N00-001
- Purity: technical grade, 99.8%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Housing: housed in suspended wire cages
- Diet: ad libitum, commercial chow (Zeigler)
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature: 24 ± 2°C
- Humidity: 45-55%
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Details on oral exposure:
Administered as 50% solution in corn oil or 5% solution in methylcellulose
Doses:
2510, 3160, 3980, 5010, 6310, 7940, 10 000 mg/kg bw
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
Statistics:
Not specified

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
7 080 mg/kg bw
Based on:
not specified
95% CL:
6 240 - 8 040
Remarks on result:
other: as 5% solution in methylcellulose
Sex:
male
Dose descriptor:
LD50
Effect level:
7 690 mg/kg bw
Based on:
not specified
95% CL:
6 380 - 9 250
Remarks on result:
other: as 5% solution in methylcellulose
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
4 460 mg/kg bw
Based on:
not specified
95% CL:
3 900 - 5 110
Remarks on result:
other: as 50% solution in corn oil
Sex:
male
Dose descriptor:
LD50
Effect level:
5 160 mg/kg bw
Based on:
not specified
95% CL:
4 250 - 6 270
Remarks on result:
other: as 50% solution in corn oil
Mortality:
See the table below for detailed results for the test material in corn oil.
Clinical signs:
Red exudate appeared around eyes at all dosages and persisted throughout 14-day observation period. Tremors, ataxia, gasping appeared in dosages of 5010 mg/kg and higher and persisted for 3 days.
Body weight:
See the table below for detailed results for the test material in corn oil.
Gross pathology:
One dead rat showed degeneration of the kidney cortex.
No gross lesions except those attributable to euthanasia were observed in survivors.

Any other information on results incl. tables




















































































































































































Dosage



Conc. % in corn oil



Onset of signs (S), mortality (M)



Cumulative mortality



Mean body weight (g)



Hours



Days



Init



Fin



 



Days



0-4



4-12



12-24



2



3



4



5



6



7



8-14



 



 



 



 



3



7



14



2510



50



 



S



 



 



 



 



 



 



 



 



0/6



212 ± 4



272 ±16



 



228 ±9



253 ±12



272 ±16



3160



 



S



 



M = 1



M = 1



 



 



 



 



 



2/6



210 ±9



272 ±10



 



221 ±12



249 ±10



272 ±10



3980



 



S



 



 



 



 



 



 



 



 



0/6



205 ±6



251 ±30



 



190 ±41



221 ±29



251 ±30



5010



 



S



M = 2



 



 



 



 



 



 



 



2/6



210 ±7



254 ±18



 



207 ±11



 227 ±13



254 ±18



6310



 



S



M = 3



M = 1



 



 



 



 



M = 1



 



5/6



210 ±4



250



 



187



217



 250



7940



 



S



M = 1



M = 3



M = 1



 



 



 



 



 



5/6



240 ±4



215



 



164



190



215



10000



 



S



M = 1



M = 2



M = 2



M = 1



 



 



 



 



6/6



205 ±6



-



 



-



-



-



 

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
Under the test conditions applied, the test material is of low acute toxicity by oral route, with a LD50 (female rats) of 4460 mg/kg bw.
Executive summary:

The acute toxicity of hexachloroethane was assessed by performing an acute oral toxicity study in Sprague-Dawley rats. There was no information regarding a standardised method or GLP.


The test material dissolved in either corn oil (50% w/v) or methylcellulose (5% w/v) was administered by stomach tube to male and female rats. The dose administered ranged from 2510 to 10 000 mg/kg bw. The oral LD50 were calculated after a 14-day observation period.


Oral LD50 were of 5160 mg/kg bw in males and 4460 mg/kg bw in females administered with the test material in corn oil. With hexachloroethane dissolved in methylcellulose, LD50 were of 7690 and 7080 mg/kg bw in males and females, respectively. Red exudate appeared around eyes at all dosages and persisted throughout 14-day observation period. Tremors, ataxia, gasping appeared in dosages of 5010 mg/kg and higher and persisted for 3 days. One dead rat showed degeneration of the kidney cortex. No gross lesions except those attributable to euthanasia were observed in survivors.


Under the test conditions applied, the test material is of low acute toxicity by oral route and is not classified according to the classification criteria of regulation (EC) 1272/2008 (CLP).