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EC number: 617-441-5 | CAS number: 83121-18-0
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-11-11 to 1985-12-13
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test using the Hprt and xprt genes)
- Version / remarks:
- 1983
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian cell gene mutation test using the Hprt and xprt genes
Test material
- Reference substance name:
- 1-(3,5-dichloro-2,4-difluorophenyl)-3-(2,6-difluorobenzoyl)urea
- EC Number:
- 617-441-5
- Cas Number:
- 83121-18-0
- Molecular formula:
- C14 H6 Cl2 F4 N2 O2
- IUPAC Name:
- 1-(3,5-dichloro-2,4-difluorophenyl)-3-(2,6-difluorobenzoyl)urea
- Test material form:
- solid: crystalline
Constituent 1
Method
- Target gene:
- The gene for HGPRT located on the X-chromosome of the V79 cells.
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Details on mammalian cell type (if applicable):
- CELLS USED
- Type and source of cells: cellbank of test laboratory
- Suitability of cells: The V79 cell line has been used for many years in experiments with success.
- Absence of Mycoplasma contamination: not specified
- Methods for maintenance in cell culture: subcultured twice a week
- Cell cycle length, doubling time or proliferation index : doubling time 12-16 h
- Modal number of chromosomes: 22
MEDIA USED
- Type and composition of media, CO2 concentration, humidity level, temperature: MEM medium supplemented with 10% fetal calf serum (FCS). Cells have been cultured at 37°C, 5% CO2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
- source of S9: rat liver
- method of preparation of S9 mix: The S9 liver microsomal fraction is obtained from the liver of 8 - 12 weeks old male Wistar rats, strain CFHB (weight ca. 150-200 g) which had been given a single i. p. injection of 500 mg/kg bw Aroclor 1254 in olive oil 5 days previously. After cervical dislocation the livers of 5 animals are removed, washed in 150 mM KCl and homogenized. The homogenate, diluted 1:3 in KCl centrifuged cold at 9000 g for 10 minutes. The supernatant which contains microsomes is frozen in ampoules of 2 or 5 mL and stored in liquid nitrogen. For standardization a protein estimation is made.
- concentration or volume of S9 mix and S9 in the final culture medium: Before the experiment an appropriate quantity of S9 supernatant is thawed and mixed with S9 cofactor solution to result in a final protein concentration of 0.3 mg/mL in the cultures. The concentration in the S9 preparation is between 30 and 45 mg/mL. The composition of the cofactor solution is concentrated to yield the following concentrations in the S9 mix: 8 mM MgCl2; 33 mM KCl; 5 mM glucose-6-phosphate; 5 mM NADP; 100 mM sodium-ortho-phosphate-buffer, pH 7.4. During the experiment the S9 mix is stored in an ice bath.
- quality controls of S9: not specified - Test concentrations with justification for top dose:
- 5, 10, 25 and 50 µg/mL
The test substance could be solubilized up to 50 µg/mL, higher concentrations precipitated in the medium. - Vehicle / solvent:
- - Solvent used: DMSO
- Justification for choice of solvent: The solvent was chosen because it proved to be relatively nontoxic in the concentrations applied.
- Justification for percentage of solvent in the final culture medium: final concentration in the medium 1 % is non-toxic.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9,10-dimethylbenzanthracene
- Remarks:
- with S9 mix, 15.4 µg/mL
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- without S9 mix, 1 mg/mL
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of independent experiments: 2
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding: 1x 10E6 cells per flask in 30 mL medium for mutagenicity test
- Test substance added in medium
TREATMENT AND HARVEST SCHEDULE:
- Exposure duration/duration of treatment: 4 hours at 37°C
Treatment scheme:
Day 1: Subculturing of a log-phase culture
a) About 400 cells in 5 mL medium/25 cm2-plastic-flask for plating efficiency; in duplicate per experimental point
b) 1 x 10E6 cells in 30 mL medium/175 cm2-plastic-flask for the mutagenicity test, 1 flask per experimental point
Day 2: Treatment of a) and b)
Day 5: Subculturing of b) in 175 cm2-plastic flasks.
Day 8: Fixation and staining of colonies in a)-flasks = plating efficiency and dose relationship
Day 9: Subculturing of b) in five 80 cm2-plastic-flasks containing selective medium: mutant selection (about 6 x 10E5 cells/flask): subculturing of b) in two 25 cm2-flasks for plating efficiency (about 500 cells/flask). For the selection of mutants the medium was supplemented with 11 µg/mL thioguanine.
Day 16: Fixation and staining of colonies in b) - derived flasks seeded on day 9.
Staining was done with 10% methylene blue in 0.01 n-KOH solution. The stained colonies with more than 50 cells were counted with a preparation.
METHODS FOR MEASUREMENT OF CYTOTOXICITY
- Method: plating efficiency - Evaluation criteria:
- The test substance is classified as mutagenic if it induces with at least one of the test substance concentrations reproducibly a mutation frequency that is three times higher than the spontaneous mutant frequency in this experiment.
The test substance is classified as mutagenic if there is a reproducible concentration - related increase in the mutation frequency. Such evaluation may be considered independently of the enhancement factor for the induced mutants.
However, in a case by case evaluation this decision depends on the level of the corresponding negative control data. If there is by chance a low spontaneous mutation rate as compared to the range normally found (10-30 mutants per 10E6 cells) a seemingly concentration-related increase of the mutations within this range will be regarded as to be irrelevant. - Statistics:
- Not necessary to perform as there is no reproducible increase of the mutation rates in the test groups treated with the test substance .
Results and discussion
Test results
- Key result
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: no cytotoxicity, but tested up to maximum solubilisable concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation and time of the determination: The test substance could be solubilized up to 50 µg/mL. Higher concentrations precipitated in the medium.
RANGE-FINDING/SCREENING STUDIES
- The toxicity of the test substance was determined in a pre-experiment by establishing the concentration related plating efficiency. According to these data the concentration range of 5, 10, 25 and 50 µg/mL was chosen in the main test with and without S9 mix.
STUDY RESULTS
- Concurrent vehicle negative and positive control data: The sensitivity of the test system was demonstrated by the enhanced mutation rates found in the groups treated with the positive control substances compared to the negative control.
- Results from cytotoxicity measurements: No cytotoxicity was detected, plating efficiency was 84.3 - 106.7% in test substance-treated cultures across the two experiments performed.
- Genotoxicity results: With no concentration of the test substance a reproducible enhancement of the mutation rate over the range of the negative controls was found, neither without nor with metabolic activation by S9 mix.
HISTORICAL CONTROL DATA: "spontaneous mutation rate [...] normally found" was given as 10-30 mutants per 10E6 cells.
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