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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
72h
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
other: ECHA website
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
not specified
Remarks:
not specified according to ECHA website for 3179-80-4

Test material

Constituent 1
Reference substance name:
Amides, coco, N-[3-(dimethylamino)propyl]
EC Number:
268-771-8
EC Name:
Amides, coco, N-[3-(dimethylamino)propyl]
Cas Number:
68140-01-2
IUPAC Name:
Amines, C12-18, alkyldimethyl

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +- 2°C
Nominal and measured concentrations:
nominal concentrations: control; 0.025; 0.05; 0.075; 0.1; 0.25; 0.5; 0.75 mg/l
Details on test conditions:
TEST SYSTEM
- Initial cells density: 10 000 cells/ ml
- Control end cells density: 271E3 cells/ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: not reported, probably yes

OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: constant illumination
- Light intensity and quality: 2000 lux with white fluorescent tubes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter at Ex 485; Em 685 nm in 1 ml cuvettes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.05 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Static acute toxicity test according to OECD TG 201 with Scenedesmus subspicatus. Test meets validity criteria of the guideline. No analytical monitoring was made. Therefore the test results are based on nominal concentration.
ErC50 (72 h) = 0.36 mg/l
ErC10 (72 h) = 0.1 mg/l
EbC50 (72 h) = 0.18 mg/l
EbC10 (72 h) = 0.05 mg/l

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