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Endpoint summary

Description of key information

Based on the available environmental fate and ecotoxicological data for daphnids, fish and algae, this substance does not needs to be classified as according to the EU CLP (Regulation (EC) No. 1272/2008).

Additional information

Studies of this substance are available for evaluating biodegradation, microbial metabolism, and toxicity to aquatic vertebrate and invertebrate species.

This substance is not readily biodegradable when tested in accordance with OECD Guideline 301B. The test shows that the substance attained 16% biodegradation after 28 days.

This substance caused a significant adverse effect on the oxygen consumption of the microbes associated with the activated sludge (OECD Guideline 209). The EC₅₀for inhibition of metabolism, as represented by respiration, was greater than 10,000 ppm. The calculated no adverse effect concentration (NOAEC) is equal 1000 ppm.

The acute aquatic toxicity of the test material was evaluated using rainbow trout and was conducted in accordance with OECD 203 and U.S. EPA 797 Guidelines. The96 hour LC50 and the NOEC were determined to be 44.35 mg/L and 33.02 mg/L, respectively. No sublethal effects were observed during the 72 hour testing period in all treatments and controls. The sublethal effect of lethargy was observed in one organism in the 0.11 mg/L test concentration at the 96 hour exposure period.

In an acute toxicity of this substance with daphnia magna (OECD Guideline 202) the 48-Hour EL*50 was 11 mg/l loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/l loading rate WAF. Chemical analysis of the test preparations at 0 (fresh media), 24 (old and fresh media) and 48 (old media) hours showed measured test concentrations to range from 0.015 mg/l to 0.265 mg/l. A concentration dependant general trend was consistently observed (with increasing loading rate) in both the fresh and old media at both media renewal periods, and a slight decline in measured concentration was consistently observed over each media renewal period. The control test sample at 0 hours showed a measured concentration of 0.0030 mg/l. Analysis of a frozen duplicate sample showed a measured concentration of less than the limit of quantification (LOQ) which confirmed the original analysis to be erroneous. The dissolved test material may have been one or several components of the test material. Because that toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole, the results were based on nominal loading rates only. The 48-Hour EC50 for the reference material to Daphnia magna based on nominal concentrations was 0.78 mg/l. The No Observed Effect Concentration was 0.32 mg/l.

In an acute study with freshwater algae that was conducted in accordance with OECD Guideline 201 and US EPA (TSC) 797.1050 the 96 hour EC₅₀for this substance was and 96 hour NOEC was 27 mg/L when calculated using cells/ml and 120 mg/l when determined using the average specific growth rate. The NOEC was 3.3 mg/L when determined using either the number of cells/ml or the average specific growth rate.

Chronic toxicity was evaluated in Daphnia (OECD guidance 211). the 21d EL50 (immobilisation) was > 10mg/L; the 21d EL50 (reproduction) was 7.8 mg/L; the 21d NOELR based on immobilisation and reproduction was 3.2 mg/L.