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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08.04.2021 - 15.06.2021
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2021
Report date:
2021

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
other: Commission Regulation (EC) No 440/2008, C.2
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine
Molecular formula:
C14H24O5N - C47H77O16N4
IUPAC Name:
Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine
Test material form:
liquid: viscous
Specific details on test material used for the study:
Name of test substance: Emuldur 3643
Test substance No.: 19/0071-2
Batch identification: 27771924U0
Purity: 100 area-% UVCB
Homogeneity: Given, visual
Storage stability: Expiry Date: 15 Jul 2021

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The following concentrations were tested: 0 (control), 4.6, 10, 22, 46 and 100 mg/L mg/L as nominal concentrations based on test substance mass without correction for content or purity.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna STRAUS 1820
- Age at study initiation (range): 2 - 24 hours
- Source: supplied by Institut National de Recherche Chimique Appliquée, France, in 1978
- Feeding during test: None

Study design

Test type:
static
Water media type:
freshwater
Remarks:
Synthetic fresh water (Elendt M4) prepared according to OECD 202.
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.20 – 3.20 mmol/L
Test temperature:
19.2 - 20.2°C
pH:
7.5 – 8.5
Dissolved oxygen:
8.0 - 9.3 mg/L
Conductivity:
550 - 650 μS/cm
Nominal and measured concentrations:
0 (control), 4.6, 10, 22, 46 and 100 mg/L as nominal concentrations based on test substance mass without correction for content or purity. The analytical recovery efficiency was 100.4 – 101.1%. The effective concentrations in this report are based on the nominal concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass vessels
- Volume of solution: 50 mL
- Renewal rate of test solution: after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The synthetic medium M4 is used for the culture and test. The medium is prepared on the basis of an ultrapure, deionized water.
- Acid capacity (up to pH 4.3): 0.80 - 1.00 mmoI/I
- Ca/mg ratio: about 4:1
- Conductivity: 550-650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: about 157 – 694 lux at a wavelength of 400 – 750 nm; 16 hours light : 8 hours darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility after 24 and 48 h

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
29.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a 48 hours static acute toxicity study with Daphnia magna the EC50 of Emuldur based on the nominal concentrations was 29.6 mg/L.
Executive summary:

In a static acute toxicity laboratory study, water flea neonates (less than 24 h old) were exposed to Emuldur 3643 at concentrations of 4.6, 10, 22, 46 and 100 mg/L in M4 test medium. Daphnids were observed for immobility at 24 and 48 hours after start of exposure.